Investigate the frequency of genes sul and investigate the relationship between genes sul and Class I integrons in Klebsiella pneumoniae isolates resistant to sulfonamides isolated from clinical cases in Shahrekord

Subject Areas : microbiology

Marziyeh  Farsinejad ¹ (M.Sc. Student of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.)
Maryam  Reisi ² (Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
JAMSHID  Alibabaeishahraki ³ ()
Hossein  Khodabandeh shahraki ⁴ ()

Received: 2024-03-31 Accepted : 2024-04-01 Published : 2024-04-01

Keywords: Integron, Klebsiella pneumonia, Multi-resistance antibiotics, Sulfonamides ,

Abstract :

Antimicrobial resistance is a common problem throughout the world. Acquire integrons is one One of the main causes multi- resistance in gram-negative microorganisms. The purpose of this study, Investigate the frequency of genes sul and investigate the relationship between genes sul and Class I integrons in Klebsiella pneumoniae isolates resistant to sulfonamides isolated from clinical cases in Shahrekord. In this study, antibiotic resistance 90 isolates of Klebsiella pneumoniae isolated from clinical cases of Shahrekord,disk diffusion method was performed. In order to investigate resistance to sulfonamides of the antibiotic cotrimoxazole was used. Then using specific primers was performed tracing genes sul1, sul2 and sul3 and intI. After PCR reaction of 33 isolates resistant to trimethoprim-sulfamethoxazole sul1 gene in 15 isolates (45/45%), sul2 gene in 20 isolates (60/60%), gene Sul3 in 2 isolates (6/06%) and the gene intI 27 isolates was found. The statistical analysis between genes sul1and IntI significant relationship was observed. The results of this study show that is a strong correlation between carry integrons and increased resistance to a number of different classes of antibiotics.

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