On the use of Response Surface Methodology for Optimization of Scaffold Composite of the Hydroxyapatite and Diopside

Subject Areas :

Nasrin Rafiee ¹ , Saeid Karbasi ² , amirabas nourbakhsh ³ , kamran amini ⁴

1 - Department of Materials Science and Engineering, Shahreza Branch, Islamic Azad University, Shahreza, Iran
2 - Department of Biomaterials and Tissue Engineering, School of Advanced Technology in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 - depCenter for Advanced Engineering Research, Majlesi Branch, Islamic Azad University, Isfahan, Iranartment
4 - Majlesi Branch, Islamic Azad University

Received: 2020-03-31 Accepted : 2020-07-09 Published : 2021-06-22

Keywords: response surface methodology, scaffold, Diopside, hydroxyapatite, Central composite design, Tissue Engineering,

Abstract :

The response surface methodology is a statistical approach to design the experiments, modeling and analysis of the effective factors as well as to help optimizing the process. In this study, we use the central composite design technique to select the optimum scaffold composite of the Hydroxyapatite and Diopside. This method suggested twenty different scaffold specimens by optimizing the suitable percentage of porosity via determining the percentage weight concentrations of the three effective parameters. After making the scaffold and determining their porosity, the optimum case for composite scaffolds was 77.57 wt% nHA (22.43wt% Di), 0.64wt% lubricant (STPP) and heat treatment temperature 1200. Also, the results of SEM, FTIR, and XRD confirm that the scaffold specimen made with the response method is an ideal specimen for use in bone tissue engineering. In general, according to the results of this research, the response surface methodology can be a useful tool for optimizing composite scaffolds in tissue engineering.

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