Synthesis and Characterizatin of Polyvinyl Alcohol/Aloe Vera Hydrogel Film by Solvent Casting Method for Wound Dressing Applications

Subject Areas :

محبوبه محمودی ¹ , مونا حاجیان ² , رعنا ایمانی ³

1 - مدیرگروه کارشناسی ارشد مهندسی پزشکی دانشگاه ازاد اسلامی واحد یزد
2 - دانشجو
3 - هیآت علمی گروه مهندسی پزشکی دانشگاه صنعتی امیرکبیر

Received: 2015-12-15 Accepted : 2017-12-10 Published : 2017-11-22

Keywords: Wound Dressing, aloe vera, Polyvinyl Alcohol, Solvent Casting, Hydrogel Films,

Abstract :

In this study, we have tried to make hydrogel films by polyvinyl alcohol and Aloe vera which used in treat wounds. The films were prepared by solvent casting method with different concentration of aloe vera include 20, 30 and 50%. Some properties of samples such as water absorption, degradation rate, water vapour transmission rate and mechanical properties were examined. Also, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) test were carried out in order to identify the bonds and morphological assessment in the samples, respectively. The results showed that with increasing of aloe vera concentration to 30% (PA30), water absorption rate, degradation rate and mechanical properties are increased. In according to SEM investigations, we found with aloe vera increasing, the porosity on surface of samples was increased. In the other hand, water vapour transmission rate have direct comparison with percentage of aloe vera concentration. Finally, cell cytotoxicity and morphology fibroblast cells (L929) attached on the samples surface evaluated by in vitro assay. The results showed that cells proliferated in greater numbers on the PA30 sample compared to other samples. Therefore, our results indicated that polyvinyl alcohol/Aloe vera hydrogel films are a good choice for use in wound dressing.

References:

 

[1]    R. F. Pereira, A. Carvalhob, A. Mendesa & P. J. Bartolo, “Influence of Aloe vera on water absorption and enzymatic in vitro degradation of alginate hydrogel filmsˮ, PubMed., Vol. 15, pp. 1-7, 2013.

 

[2]    S. Moritz, C. Wiegand, F. Wesarg, N. Hessler, F. A. Müller, D. Kralisch, U. C. Hipler & B. Fischer, “Active wound dressings based on bacterial nanocellulose as drug delivery system for octenidineˮ, International Journal of Pharmaceutics, Vol. 471, pp. 1-4, 2014.

 

[3]    C. Radhakumarya, M. Antontyb & K. Sreenivasan, “Drug loaded thermoresponsive and cytocompatible chitosan based hydrogel as a potential wound dressingˮ, Carbohydrate Polymers, Vol. 83, pp. 1-8, 2010.

 

[4]    G. Zhou, A. Ruhan, Ge. Heng, L. Wang, M. Liu, B. Wang, H. Su, M. Yan, Y. Xi & Y. Fan, “Research on a novel poly (vinyl alcohol)/lysine/ vanillin wound dressing: Biocompatibility, bioactivity and antimicrobial activityˮ, BURNS, Vol. 4346, pp. 1-9, 2014.

 

[5]    R. Pereira, A. Mendes & P. Bártolo, “Alginate/Aloe vera hydrogel films for biomedical applicationsˮ, The First CIRP Conference on Biomanufacturing, Vol. 5, pp. 1-6, 2013.

 

[6]    B. Singha, S. Sharmab & A. Dhiman, “Design of antibiotic containing hydrogel wound dressings: Biomedical properties and histological study of wound healingˮ, International Journal of Pharmaceutics, Vol. 457, pp. 1-7, 2013.

 

[7]    G. D. Mogoşanu & A. M. Grumezescu, “Natural and synthetic polymers for wounds and burns dressingˮ, Pediatric nephrology, Vol. 29, pp. 1, 2013.

 

[8]    L. Vandamme, A. Heyneman, H. Hoeksema, J. Verbelen & S. Monstrey, “Honey in modern wound care: A systematic reviewˮ, BURNS, Vol. 4076, pp. 1- 7, 2013.

 

[9]    J. O. Kimb, J. K. Parka, J. H. Kimb, S. Giu Jin, C. S. Yonga, Li D. Xun, J. Y. Choia & J. S. Wooa, “Development of polyvinyl alcohol–sodium alginate gel-matrix-based wound dressing system containing nitrofurazoneˮ, International Journal of Pharmaceutics, Vol. 359, pp. 1-4, 2008.

 

[10] S. Jettanacheawchankit, S. Sasithanasate, P. Sangvanich, W. Banlunara, P. Thunyakitpisal5, “Acemannan Stimulates Gingival Fibroblast Proliferation, Expressions of Keratinocyte Growth Factor-1, Vascular Endothelial Growth Factor, and Type I Collagen; and Wound Healingˮ, Journal of Pharmacological Sciences, Vol. 109, pp. 1-4, 2009.

 

[11] J. O. Kimb, J. K. Parka, J. H. Kimb, S. Giu Jin, C. S. Yonga, Li. D. Xun, J. Y. Choia, J. S. Wooa, B. K. Yooa, W. Seok Lyooc, J. A. Kima & H. G. Choia, “Development of polyvinyl alcohol–sodium alginate gel-matrix- based wound dressing system containing nitrofurazoneˮ, International Journal of Pharmaceutics, Vol. 359, pp. 1-4, 2008.

 

[12] B. Hui, Zhang & Y. L. Ye, “Preparation of PVA Hydrogel Beads and Adsorption Mechanism for Advanced Phosphate Removalˮ, Chemical Engineering Journal, Vol. 74, pp. 3-7, 2014.

 

[13] R. Pereira, A. Mendes & P. Bártolo, “Alginate/Aloe vera hydrogel films for biomedical applications, The First CIRP Conference on Biomanufacturingˮ, Vol. 5, pp. 1-6, 2013.

 

[14] R. F. Pereira, A. Carvalhob, A. Mendesa & P. J. Bartolo, “Influence of Aloe vera on water absorption and enzymatic in vitro degradation of alginate hydrogel filmsˮ, PubMed, Vol. 15, pp. 1-7, 2013.

 

[15] S. S. Silva, E. G. Popa, M. E. Gomes, M. Cerqueira, A. P. Marques, S. G. Caridade, P. Teixeira, C. Sous, J. F. Mano & R. L. Reis, “An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicineˮ, Acta Biomaterialia, Vol. 49, pp. 1-7, 2013.

 

[16] S. Khoshgozaran Abrasa, M. H. Azizi, Z. Hamidyan & N. Bagheripoor Fallahb, “Mechanical, physicochemical and color properties of chitosan based-films as a function of Aloe vera gel incorporationˮ, Carbohydrate Polymers, Vol. 87, pp. 2-5, 2011.

 

[17] P. Inpanya, A. Faikrua, A. Ounaroon, A. Sittichokechaiwut & J. Viyoch, “Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin induced diabetic rats, Biomedˮ, Vol. 7, pp. 1-8, 2012.

 

[18] P. Jithendra, A. Merlin Rajam, T. h. Kalaivani, A. Mandal & C. h. Rose, “Preparation and Characterization Of Aloe Vera Blended Collagen- Chitosan Composite Scaffold for Tissue Engineering Applicationsˮ, International Journal of Pharmaceutic, Vol. 97, pp. 2-7, 2013.

 

[19] Uslu, S. Keskin, A. Gül, T. C. Karabulut & M. L. Aksu, “Preparation and Properties of Electrospun Poly (vinyl alcohol) Blended Hybrid Polymer with Aloe vera and HPMC as Wound Dressingˮ, Journa of biology and chemestery, Vol. 38, pp. 1-5, 2009.

 

[20] H. E. Thu, M. Zulfakar & S. h. Fern, “Alginate based bilayer hydrocolloid films as potential slow-release modern wound dressingˮ, International Journal of Pharmaceutics, Vol. 434, pp. 1-8, 2012.

 

[21] Rayll & D. Gupta, “A panoptic study of antioxidant potential of foliar gel at different harvesting regimens of Aloe veraˮ, Materials Science and Engineering, Vol. 21, pp. 2-4, 2013.

 

[22] M. Shemesh & M. Zilberman, “Structure–property effects of novel bioresorbable hybrid structures with controlled release of analgesic drugs for wound healing applicationsˮ, Acta Biomater,Vol. 10, pp. 2-4, 2013.

 

[23] M. Kokabi, M. Sirousazar & Z. Muhammad Hassan, “PVA–clay nanocomposite hydrogels for wound dressingˮ, European Polymer Journal, Vol. 43, pp. 1- 8, 2007.

 

[24] S. Khoshgozaran Abrasa, M. H. Azizi, Z. Hamidyan & N. Bagheripoor Fallahb, “Mechanical, physicochemical and color properties of chitosan based-films as a function of Aloe vera gel incorporationˮ, Carbohydrate Polymers, Vol. 87, pp. 2-5, 2011.

 

[25] R. Pereira, A. Tojeira, C. Daniela, A. Mendes & P. Ba´rtolo, “Preparation and characterization of film Based on alginate and aloe veraˮ, International Journal of Polymer Anal Charact, Vol. 16, pp. 2-16, 2011.

 

[26] S. Massumi, “Protocol for Fixation of cells on scaffold for SEMˮ, 2012.

 

[27] G. Simbula, C. Dettori, T. Camboni & E. Cotti, “Comparison of Tetraacetylethylendiamine + Sodium Perborate and Sodium Hypochlorite Cytotoxicity on L929 Fibroblastsˮ, Basic Research-Biology, Vol. 36, pp. 1-4, 2010.

 

[28] M. Boncler, M. Różalski, A. Krajewska, A. Podsędek & C. Watala, “Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cellsˮ, Basic Research-Biology, Vol. 88, pp.1-7, 2013.

 

[29] Raya & S. h. Aswatha, “An analysis of the influence of growth periods on physical appearance and acemannan and elemental distribution of Aloe vera Lgelˮ, Acta Biomaterialia, Vol. 54, pp. 2-7, 2013.

 

[30] Singh & L. Pal, “Sterculia crosslinked PVA and PVA-poly (AAm) hydrogel wound dressings for Slow drug delivery: Mechanical, mucoadhesive, biocompatible and permeability propertiesˮ, Journal of themechanical behavior of biomedical materials, Vol. 9, pp. 2-8, 2012.

 

[31] O. Saibuatong & M. Phisalaphong, “Novo aloe vera–bacterial cellulose composite film from biosynthesisˮ, Polymer Sciences, Vol. 11, pp. 2-6, 2009.

 

[32] P. T. Alexy, A. Bakoš, G. Crkoňová, Z. Kramárováa, J. Hoffmannc, M. Julinovác, M. Chiellinib & P. Cinellib, “Poly(vinyl alcohol)–collagen hydrolysate thermoplastic blends: II. Water penetration and biodegradability of melt extruded filmsˮ, Polymer Testing, Vol. 22, pp. 1-3, 2003.

 

[33] D. Archanaa, J. Duttab & P. K. Duttaa, “Evaluation of chitosan nano dressing for wound healing: Characterization, in vitro and in vivo studiesˮ, International Journal of Biological Macromolecules, Vol. 57, pp. 1-8, 2013.

 

[34] H. Y. Kim, H. N. Kim, S. J. Lee, J. E. Song, S. Y. Kwon, J. W. Chung, D. Lee & G. Khang,“Effect of Pore sizes of PLGA scaffolds on mechanical properties and cell behaviour for nucleus pulposus regeneration in vivoˮ, Tissue Eng. Regen. Med., Vol. 11, pp. 1-8, 2014.

 

[35] K. M. Abdullah, A. Abdullah, M. L. Johnson, J. J. Bilski, K. Petry, D. A. Redmer, L. P. Reynolds & A. T. Grazul-Bilska, “Effects of Aloe vera on gap junctional intercellular communication and proliferation of human diabetic and nondiabetic skin fibroblastsˮ, The Journal of Alternative & Complementary Medicine, Vol. 9, pp. 711-718, 2003.

 

S. Silva, E. G. Popa, M. E. Gomes, M. Cerqueira, A. Marques, S. Caridade, P. Teixeira, C. Sousa, J. ano & R. Reis, “An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicineˮ, Acta biomaterialia, Vol. 9, pp. 6790-6797, 2013.