Investigation of mechanical and biological properties of functionalized polycaprolactone and polyethylene glycol diacrylate based scaffold reinforced with hydroxyapatite

Subject Areas :

نرجس  کوپائی ¹ (استاد دانشگاه/دانشگاه آزاد اسلامی واحد نجف آباد)
Akbar  Karkhaneh ² (استاد دانشگاه/دانشگاه صنعتی امیر کبیر)

Received: 2016-10-29 Accepted : 2018-12-18 Published : 2018-11-22

Keywords: scaffold, hydroxyapatite, polycaprolactone diacrylate, polyethylene glycol diacrylate, crosslink,

Abstract :

The purpose of this study is synthesised of functionalized polycaprolactone and polyethylene glycol diacrylate based scaffold in the presence of hydroxyapatite (HA) particles and investigated of mechanical and biological properties of scaffolds. . At the first step, PCL diol was acrylated with acryloyl chloride and confirmed using fourier transform infrared (FTIR).Then, the scaffolds were synthesized by radical crosslinking reaction of polycaprolacton acrylate and polyethylene glycol diacrylate (PEGDA) in the presence of hydroxyapatite (HA) particles and particulate technique with sodium chloride. The prepared samples were characterized using techniques such as scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), and dynamic mechanical thermal analysis (DMTA). Results show that increasing the ratio of PEGDA to polycaprolactone diacrylate (PCLDA) led to increase of tan δ peak and decrease of compressive modulus of the network, respectively. It was found that the incorporation of HA particles with the polymer matrices resulted in an increased of compressive modulus and a decrease of tan δ peak. Cytocompatability of the scaffolds were assessed by direct contact test and staining cell. Results indicated no toxicity, and cells attached and spread on the pore walls offered by the scaffols. Thus, the results indicated that the PCLDA/PEGDA/HA scaffolds have the potential of being used as promosing substrates in bone tissue engineering.

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