Evaluation Pedigree and Morphological Traits Studies of Bread Wheat (Triticum aestivum L.) Genotypes Using RGA to Introduce Compatible Cultivars

Subject Areas :

Alireza Habibzadeh ¹ , Mohammad Khayat ²

1 - Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
2 - Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.

Received: 2016-09-11 Accepted : 2017-01-01 Published : 2018-11-22

Keywords: Morphology, Bread Wheat, Genotypes, Pedigree, RGA technique,

Abstract :

In this study 30 varieties of wheat genetic diversity inside and outside using RGA markers was investigated. Delaporta method using DNA samples were extracted from the leaves. 5 pairs of RGA primers used three primer pairs showed polymorphism. The highest and lowest number of polymorphism markers in order to The P14N and P54N. Percent band polymorphism markers P810, P54N and P14N Respectively 42%, 33% and 39% respectively. Cluster analysis based on presence and absence of bands using Jaccard's similarity coefficients were based UPGAMA. Range of similarity coefficients ranged from 0.061 to 0.88. Most similarities between the Kerman and Kuhdasht variety but least similarity between cultivars TP981, Lee was. In this study a significant relationship between genetic distance and geographical distance was observed in culture, so that Iranian and foreign cultivars with 0.44 similarity coefficients were separate. But two varieties Anza and Hyrmand this division did not comply. Also, the position of the genetic diversity of Iranian figures (with pedigree unknown) function in the cluster was their kinship relations. All yellow rust sensitive cultivars were all placed in the lower part of the dendogram. The results were indicative of the efficacy of RGA technique for evaluation variation of resistance analogues