Investigation of broiler chicken Spirochetosis in urmia city
Subject Areas : Pultry Diseases
Gunash Daneshghar
1
,
Mehdi Rezaei
2
*
,
Mohamad Reza Asgharzadeh
3
1 - 1- Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
2 -
3 - Department of Biology, Faculty of Basic Science, Ur.C.,, Islamic Azad University, Urmia, Iran.
Keywords: Spirochetes, broiler chicken, Tracheal Swab, Gastrointestinal Swab, Giemsa Staining, PCR,
Abstract :
Spirochetosis is a bacterial disease in poultry caused by spirochetes. The disease is more prevalent in flocks raised in unsanitary conditions and can cause serious problems in the respiratory and digestive systems of birds. The aim of the present study was to investigate the prevalence and isolation of spirochete bacteria from tracheal and gastrointestinal swabs of broiler chickens in Urmia city. For this purpose, 75 tracheal swab samples and 75 intestinal swab samples were randomly collected from 15 broiler flocks and subjected to Giemsa staining and polymerase chain reaction (PCR) tests. In the first step, microscopic examinations were performed with Giemsa staining. To confirm the results, PCR using specific primers was used. All samples were negative in Giemsa staining. Despite DNA extraction and primer design for a 230-bp fragment, none of the samples tested positive for spirochetes. The findings of this study indicate the absence of spirochetes in broiler poultry in Urmia County. These results, which are presented as the first study in the region, can be used as basic information in assessing the health status of broiler poultry breeding units. Also, considering the lack of detection of spirochetes in the samples, it indicates the favorable conditions of biosecurity in the farms of the region. Finally, the use of more sensitive and advanced methods such as PCR can be an effective tool for more accurate identification of bacterial diseases and infections in the poultry industry in the future.
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