Molecular Identification of Quinolone Resistance Genes (aac (6 ') - ib-cr and qnrS) and Determination of Antibiotic Resistance Pattern of Salmonella Isolated from Laying Poultry of East Azarbaijan Province

Subject Areas : Pultry Diseases

hossein nikpiran ¹ , younes anzabi ² , abdolrahman mohammadi ³

1 - Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
2 - Assistant Professor, Department of pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
3 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

Received: 2019-10-20 Accepted : 2021-06-10 Published : 2020-04-20

Keywords: East Azarbaijan, Salmonella, Antibiogram, Genetic resistance,

Abstract :

The use of antibiotics in the treatment of salmonellosis is of particular interest both in veterinary medicine and in medicine. Due to the widespread use of antibiotics in the therapeutic debate, resistance to these agents has increased among bacteria. The aim of this study was to evaluate the presence of quinolone resistance genes and to determine the antibiotic resistance pattern of Salmonella isolates from laying poultry. For this purpose, in a period of approximately three months in 1977, 20 cases of reference laying hens were sampled from different farms with symptoms of Salmonella. Biochemical tests were used to identify the isolates and to determine the serotype by the Kauffman White method for the identification of somatic O antigen and flagella H. After serotyping in the Muller Hinton Agar environment, antibiogram tests were performed using the disks of anrofloxacin, phosphomycin, danofloxacin, chlortetracycline, oxytetracycline, doxycycline, soltreim, erythromycin and amoxicillin. After determination of qnrs and aac (6 ') - ib-cr resistance genes, the respective primers were designed. Bacterial genes were extracted by boiling and the presence of the genes was evaluated by PCR. Positive samples indicate the presence of quinolone resistance genes in Salmonella isolates from laying poultry. The findings of this study indicate the risk of resistance in herds in the province and highlight the importance of addressing this issue by national health authorities.