The study of histoanatomical and S-100 gene expression in Japanese quail (Coturnix coturnix japonica) testes
Subject Areas : Histology
1 - Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Keywords: Immunohystochemistry, quail, Testis, Gene Expression,
Abstract :
Testicular tissue is one of the most important and sensitive tissues of the body. The main role of testis is to produce sperm and testosterone. So far, research into immunohistochemical staining of S-100 protein has not been performed in Japanese testicular tissue. The aim of this study was to evaluate the topography, histoarchitecture and S-100 protein localization in Japanese quail testicles. 8 male Japanese quail were selected which weighing 4.72± 196.167 g and had the identical feeding and breeding conditions. The testicles were studied after stages of the tissue passage to examine the S-100 protein and histology under light microscope. The T-test was used in order to analyze the data at significant level of P< 0.05. our results showed that the right testis of the Japanese quail was bean-shape and the left one was spherical. Histological results demonstrated that there was a severe decrease of interstitial tissue of testis in some of the samples as well as the existence of 10-12 cellular layers in the walls of the seminiferous tubules. S-100 protein was not observed in seminiferous tubules. obtained results exhibited the structure of testicular tissue was the same as the other birds topographically and histologically, although the right testis of the quail was completely bean-shaped. In histological studies of the quail’s testis, the connective interstitial tissue was one of the significant properties. the evaluation of testis immunohistochemical staining S-100 protein was not observed in the structural of seminiferous tubules, sexual, leydig cell, interstitial tissue, straight tubules and rete testis.