The effect of heat stress on some blood parameters, biochemical values and humoral immunity in Broiler Chickens
Subject Areas :
Veterinary Clinical Pathology
عادل Feizi
1
,
فرهاد Dadian
2
,
ساجد Asadzadehmajdi
3
1 - Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 - BVLSc, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
Received: 2012-07-24
Accepted : 2012-10-25
Published : 2012-11-21
Keywords:
broiler chickens,
heat stress,
blood parameters,
biochemical,
Humoral immune,
Abstract :
This research was conducted to determine the effects of Heat stress on some Blood Parameters, serum Biochemical values and humoral immunity in broiler chicks. Eighty day old Cobb broilers were randomly divided into 2 equal groups of control and treatment. Both groups were kept under the same management, nutrition, density, ventilation and vaccination conditions. The only difference between the two groups was treatment of the last week of the breeding period (days 36-42). Which was 24 0C in the control group and 33 0C in the treatment group. At the end of the breeding period (days 42), blood samples were taken from the brachial vein of all broiler. To determine the blood parameters, part of the samples were collected in anticoagulant containing capillary tubes (1mg/ml, EDTA) and were determined immediately. The Haematocrit, Leucocyte counts and differential Leucocyte counts were determined immediately. The other portions of the brachial blood were used to determine blood PH and following centrifuging and separation of the sera, some biochemichal parameters such as Total protein and Glocose were evaluated by colorimetric method with specific kits. To determine the effect of heat stress on Humoral immiunity, serum samples were tested with HI (Haemagglutination Inhibition) method to to clarify antibody production in response to Newcastles disease vaccine. The results of this study showed that Haematocrit, Lymphocyte percentage and Total protein were significantly decreased (p<0/05), but serum levels of Glucose, blood PH, Heterophil percentage H/L and ratio was significantly increased in the treatment group (p<0.05). Evaluation of the Monocyte and Eosinophil percentage did not reveal statistically significant differences between the treatment and control groups (p>0.05). Results of Newcastle HI test showed that HI titers were significantly decreased in treatment group (p<0.05). As a result, broiler chicks subjected to heat stress are susceptible to Newcastles disease and subsequent secondary infections due to suppression of the immune system.
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