Evaluation of mice infected to Salmonella Spp in Poultry farms of Tehran Province

Subject Areas : Veterinary Clinical Pathology

مرتضی Hadadian ¹ , وحید Karimi ² , تقی Zahraee Salehi ³ , عباس Barin ⁴ , آرش Ghalyanchi Langeroudi ⁵

1 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
2 - Department of Poultry Diseases, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
3 - Department of Microbiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
4 - Department of Patobiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
5 - Department of Microbiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

Received: 2011-12-31 Accepted : 2012-10-25 Published : 2012-08-22

Keywords: Mice, Salmonella sp, Poultry Farm,

Abstract :

In this survey, 290 mice and rats fecal samples from commercial layer and broiler poultry houses were tested for Salmonella sp. presence. All samples were cultured on Selenite F broth media and passaged on SS agar and McConkey agar. The suspected colonies were cultured on Urea and TSI agars to be confirmed as Salmonella sp.. Finally, Salmonella isolates were identified genetically and biochemically by PCR and conventional methods, respectively. Serogrouping and Antibiotic resistance profiling were done for further differentiation of isolates. Twenty eight (9.65%) Salmonellas were isolated from (out of) 290 samples. Eight (28.6%), seven (25%), four (14.3%), and two (7.2%) isolates were located in serogroups C, D, B and E, respectively. Seven isolates (25%) belonged to Arizona subspecies and just one non-motile serogroup D Salmonella was isolated. All isolates were sensitive to enrofloxacin, difloxacin, norfloxacin, chloramphenicol and florfenicol, but they were resistant to sulfamethoxazole, trimethoprim and neomycin in decreasing order. In addition to former surveys, this study confirmed the role of mice and rats in spreading of Salmonella spp. in poultry farms. In conclusion it is essential to take appropriate measurements (measures) for pest management in poultry houses to approach the prevention of some bacterial infection like  (such as ) salmonellosis.   

References:

1. Coburn, B., G. and A. Grassl Finlay Salmonella, the host and disease: a brief review. Immunol Cell Biol 85:112-118. 2007.


2. Montville, T. J., and K. R. Matthews Food microbiology: an introduction. ASM Press, Washington, D.C. 2005.


3. Jordan, F. T. W., and M. Pattison Poultry diseases. Bailliere Tindall. 2001.


4. Henzler, D. J., and H. M. Opitz The role of mice in the epizootiology of Salmonella enteritidis infection on chicken layer farms. Avian Dis 36:625-631. 1992.


5. Murray, P. R., and E. J. Baron Manual of clinical microbiology, 9th ed. ASM Press, Washington, D.C. 2007.


6. Guard-Petter, J., D. J. Henzler, M. M. Rahman, and R. W. Carlson On-farm monitoring of mouse-invasive Salmonella enterica serovar enteritidis and a model for its association with the production of contaminated eggs. Appl Environ Microbiol 63:1588-1593. 1997.


7. Shimi, A., M. Keyhani, and K. Hedayati Studies on salmonellosis in the house mouse, Mus musculus. Lab Anim 13:33-34. 1979.


8. Najar Peerayeh, S., N. Soleimani1, J. Sadrai2, and S. Derakhshan1 Investigation of Contamination of Wild Rats (Rattus rattus) from Tehran City to Antibiotic Resistant Enterobacteriaceae in 2009. J Mazand Univ Med Sci  20:70-75 2010.


9. Khalil, A. M. The incidence of organisms of the Salmonella group in wild rats and mice in Liverpool. J Hyg (Lond) 38:75-78. 1938.


10. Friedman, R. L., and R. J. Moon Hepatic clearance of Salmonella typhimurium in silica-treated mice. Infect Immun 16:1005-1012. 1977.


11. Vanhooser, S. L., and R. D. Welsh Isolation of Salmonella species from ratites. J Vet Diagn Invest 7:268-269. 1995.


12. Mutalib, A., P. McDonough, S. Shin, V. Patten, and D. Lein Salmonella enteritidis in commercial layer farms in New York State; environmental survey results and significance of available monitoring tests. J Vet Diagn Invest 4:416-418. 1992.


13. Badi, M. A., N. Iliadis, K. Sarris, and E. Artopios [Salmonella infection sources in poultry flocks in northern Greece]. Berl Munch Tierarztl Wochenschr 105:236-239. 1992.


14. Davies, R. H., and C. Wray Mice as carriers of Salmonella enteritidis on persistently infected poultry units. Vet Rec 137:337-341. 1995.


15. al-Nakhli, H. M., Z. H. al-Ogaily, and T. J. Nassar Representative Salmonella serovars isolated from poultry and poultry environments in Saudi Arabia. Rev Sci Tech 18:700-709. 1999.


16. Davies, R. H., and M. Breslin Persistence of Salmonella enteritidis phage type 4 in the environment and arthropod vectors on an empty free-range chicken farm. Environ Microbiol 5:79-84. 2003.