Effect of olive leaf alcoholic extract on renal ischemia/reperfusion injury in adult male rats
Subject Areas : Veterinary Clinical Pathologymohammadreza nasirzade 1 , miralireza Nourazar 2 , leila Roshangar 3
1 - دانشگاه آزاد اسلامی، واحد تبریز، استادیارگروه فیزیولوژی، تبریز، ایران.
2 - دانشگاه آزاد اسلامی، واحد تبریز، استادیار گروه فیزیولوژی، تبریز، ایران.
3 - دانشگاه علوم پزشکی تبریز، دانشیار بافتشناسی دانشکده پزشکی، تبریز، ایران.
Keywords: Antioxidant enzymes, Rat, kidney, Ischemic-reperfusion injury,
Abstract :
Ischemia-reperfusion (I/R) is present at various degrees in kidney transplants. Several studies suggest that renal ischemia reperfusion (RIR) can induce acute kidney injury. Liver diseases and neurological disorders related to kidney injury is a common clinical problem. Olive leaf is a significant source of bioactive phenolic compounds. They have better antioxidant capacity, anti-inflammatory and radical scavenging. In this study 50 male rats were allocated randomly into 5 groups: control (intact animals), group-1(I/R 60min+olive leaf extract), group-2 (I/R 60min), group-3(I/R 120min+olive leaf extract)and group-4(I/R 120min).The animals received 100 mg/kg olive leaf extract in0.5 ml drinking water using gavage for 28 days. Other animals received 0.5 ml normal saline by gavages. At the end of the treatment, the level of antioxidant enzymes including TAC, MDA, SOD and GPX were determined in renal tissue. Administration of olive leaf extract can significantly increase activity of TAC, GPX and SOD in group1and 3compared with group2and4. Also, MDA level in renal tissue of treated groups was significantly lower than ischemia-reperfusion groups (p<0.05). This study showed that olive leaf extract has protective effects against renal ischemic-reperfusion injury.
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