Effect of Coenzyme Q10 on testicular tissue after correction of experimental cryptorchidism in rat
Subject Areas :
Veterinary Clinical Pathology
Arash Shekari
1
(Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)
Esmail Safavi
2
(Assistant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran. Assistant Professor, Department of Basic Sciences, Biotechnology research center, Tabriz Branch,)
Ghafour Mousavi
3
(Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.)
Received: 2018-06-05
Accepted : 2020-09-27
Published : 2020-08-22
Keywords:
Coenzyme Q10,
Testis,
Rat,
Cryptorchidism,
orchidopexy,
Abstract :
Perturbation of testicular descent into the scrotum results in cryptorchidism. After surgical correction of cryptorchidism (orchidopexy), recovery of damaged testicular tissue is observed gradually. The aim of the present study was to histologically evaluate the effect of Coenzyme Q10 on testicular tissue after orchidopexy in the rat. In this study, 40 male immature rats were randomly divided into 4 groups of 10 animals each. The first group received no surgical intervention. Experimental bilateral cryptorchidism was induced surgically in the second group and rats were kept until the end of the experiment. In the third and fourth groups, orchidopexy was performed 35 days after induction of experimental bilateral cryptorchidism. Rats in the third and fourth group were administered oral olive oil and coenzyme Q10 respectively for 30 days after orchidopexy. At the end of the experimental period, testicular tissue samples were obtained for morphologic and histomorphometerical studies. The results were analyzed with one-way ANOVA and Tukey test and p < /em>p < /em><0.05). This study indicated that the use of Coenzyme Q10 causes testicular tissue repair and improves histomorphometrical and spermatogenesis coefficients after orchidopexy in the rat.
References:
Akunna, G., Oluwaseyi, S., Saalu, C., Babatunde, O., Godson, G. and Ayomide, J. (2012). Laurus nobilis extract preserves testicular functions in cryptorchid rat. World Journal of Life Sciences and Medical Research, 2(2): 91-98.
Babaei, H., Azari, O., Kheirandish, R., Abshenas, J. and Mohammadi, N. (2010). Zinc therapy improves deleterious effects of experimental unilateral cryptorchidism: Histopathological evaluation of testes. Iranian Journal of Veterinary Surgery, 5(12): 77-88.
Bentinger, M., Dallner, G., Chojnacki, T. and Swiezewska, E. (2003). Distribution and breakdown of labeled coenzyme Q10 in rat. Free Radical Biology & Medicine, 34(5): 563-575.
Billig, H. and Beumer, T. (1995). Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stage. Endocrinology, 136(1): 5-12.
Dellman, H.D. and Eurell, J. (1998). Textbook of veterinary histology. 5th ed., U.S.A., Williams and Wilkins, pp: 228-233.
Dhanasekaran, M. and Ren, J. (2005). The emerging role of coenzyme Q10 in aging neurodegeneration,cardiovascular disease,cancer and diabetic mellitus. Current Neurovascular Research, 2(5): 447-459.
Echtay, K.S., Winkler, E. and Klingenberg, M. (2000). Coenzyme Q is an obligatory cofactor for uncoupling protein function. Nature, 408(6812): 609-613.
El-sayed, K.A. and Gamal, H.A. (2011). Protective effect of coenzyme Q10 on cadmium-induced testicular damage in male rabbits. American-Eurasian Journal of toxiological Sciences, 3(3): 153-160.
Fouad, A., Al-Sultan, A. and Yacoubi, M. (2011). Coenzyme Q10 counteracts testicular injury induced by sodium arsenite in rats. European Journal of Pharmacology, 655(1-3): 91-98.
Gille, L. and Nohl, H. (2000). The existence of a lysosomal redox chain and the role of ubiquinone. Archives of Biochemistry and Biophysics, 375(2): 347-354.
Gracia, R., Ferrandez, L. and Alvarez, F. (1990). Experimental cryptorchidism in the Wistar rat. Cirugia Pediatrica, 3(3): 97-102.
Jegou, B., Peake, R., Irby, D. and Kretser, D. (1984). Effects of the induction of experimental cryptorchidism and subsequent orchidopexy on testicular function in immature rats. Biology of Reproduction, 30(1): 179-187.
Kaki, T. and Sofikitis, N. (1999). Effects of unilateral cryptorchidism on contralateral sperm quality quantity and fertilizing capacity. Yonago Acta Medica, 42: 79-86.
Kerr, B., Rich, K. and Fekretser, F. (1979). Alteration of the fine structure and androgen secration of the interstitial cells in the experimental cryptorchid rat testis. Biology of Reproduction, 20(3): 409-422.
Liamara, R., Luis, A. and Fabio, F. (2005). Sperm retrival techniques in rats with suppressed spermatogenesis by experimental cryptorchidism. Human Reproduction, 20(2): 443-447.
Mazen, N.F. and Elnegris, H.M. (2013). Role of coenzyme Q10 in testicular damage induced by acrylamide in weaned albino rats: a histological and immunohistochemical study. The Egyptian Journal of Histology, 36(1): 164-174.
Meistrich, M., Wilson, G. and Porter, K. (2003). Restoration of spermatogenesis in DBCP–treated rats by hormone suppression. Toxicological Sciences, 76(2): 418-426.
Mendis, S. and Kerr, J. (1990). Experimental cryptorchidism in the adult mouse: qualitative and quantitative light microscopic morphology. Journal of Andrology, 11(6): 539-547.
Nishimura, A., Fujimura, M., Hasegawa, F. and Nobuhito, S. (2010). Pharmacokinetic interaction between nifedipine and coenzyme Q10 in rat. Journal of Health Science, 56(3): 310-320.
Pinart, E., Sancho, S. and Briz, M. (1999). Morphologic study of the testis from spontaneous unilateral and bilateral abdominal cryptorchid boars. Journal of Morphology, 239(3): 225-243.
Ren, L., Medan, M. and Ozu, M. (2006). Effects of experimental cryptorchidism on sperm motility and testicular endocrinology in adult male rats. The Journal of Reproduction and Development, 52(2): 219-228.
Royere, D., Guerif, F., Laurent, V. and Hochereau de Reviers, M. (2004). Apoptosis in testicular germ cells., International Congress Series, 1266: 170-176.
Saalu, L., Oluyemi, K. and Omotuyi, I. (2007). α-Tocopherol (vitamin E) attenuates the testicular toxicity associated with experimental cryptorchidism in rat. African Journal of Biotechnology, 6(12): 1373-1377.
Sun, I.L., Sun, E.E., Crane, F.L., Morre, D.J., Lindgren, A. and Low, H. (1992). Requirement for coenzyme Q in plasma membrane electron transport. Proceedings of the National Academy of Sciences of the United States of American, 89(23): 11126-11130.
Vigodner, M. and lewin, L. (2003). Evaluation of damage to the testicular cells of golden hamsters caused by experimental cryptorchidism using flow cytometry and confocal microscopy. International Journal of Andrology, 26(2): 84-90.
Villalba, J.M., Lopez-Lluch, G., Santos-Ocana, C., Rodriguez-Aguilera, J.C. and Navas, P. (2001). Extramitochondrial functions of coenzyme Q. In: Coenzyme Q: molecular mechanisms in health and disease. The American Journal of Clinical Nutrition,73(6):1116-1117
Walter, L., Miyoshi, H., Leverve, X., Bernard, P. and Fontaine, E. (2002). Regulation of the mitochondrial permeability transition pore by ubiquinone analogs. A progress report. Free Radical Research, 36(4): 405-412.
Yin, Y., Hawkins, K. and Dewolf, W. (1997). Heat stress cause testicular germ cell apoptosis in adult mice. Journal of Andrology, 18(2): 159-165.
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Akunna, G., Oluwaseyi, S., Saalu, C., Babatunde, O., Godson, G. and Ayomide, J. (2012). Laurus nobilis extract preserves testicular functions in cryptorchid rat. World Journal of Life Sciences and Medical Research, 2(2): 91-98.
Babaei, H., Azari, O., Kheirandish, R., Abshenas, J. and Mohammadi, N. (2010). Zinc therapy improves deleterious effects of experimental unilateral cryptorchidism: Histopathological evaluation of testes. Iranian Journal of Veterinary Surgery, 5(12): 77-88.
Bentinger, M., Dallner, G., Chojnacki, T. and Swiezewska, E. (2003). Distribution and breakdown of labeled coenzyme Q10 in rat. Free Radical Biology & Medicine, 34(5): 563-575.
Billig, H. and Beumer, T. (1995). Apoptosis in testis germ cells: developmental changes in gonadotropin dependence and localization to selective tubule stage. Endocrinology, 136(1): 5-12.
Dellman, H.D. and Eurell, J. (1998). Textbook of veterinary histology. 5th ed., U.S.A., Williams and Wilkins, pp: 228-233.
Dhanasekaran, M. and Ren, J. (2005). The emerging role of coenzyme Q10 in aging neurodegeneration,cardiovascular disease,cancer and diabetic mellitus. Current Neurovascular Research, 2(5): 447-459.
Echtay, K.S., Winkler, E. and Klingenberg, M. (2000). Coenzyme Q is an obligatory cofactor for uncoupling protein function. Nature, 408(6812): 609-613.
El-sayed, K.A. and Gamal, H.A. (2011). Protective effect of coenzyme Q10 on cadmium-induced testicular damage in male rabbits. American-Eurasian Journal of toxiological Sciences, 3(3): 153-160.
Fouad, A., Al-Sultan, A. and Yacoubi, M. (2011). Coenzyme Q10 counteracts testicular injury induced by sodium arsenite in rats. European Journal of Pharmacology, 655(1-3): 91-98.
Gille, L. and Nohl, H. (2000). The existence of a lysosomal redox chain and the role of ubiquinone. Archives of Biochemistry and Biophysics, 375(2): 347-354.
Gracia, R., Ferrandez, L. and Alvarez, F. (1990). Experimental cryptorchidism in the Wistar rat. Cirugia Pediatrica, 3(3): 97-102.
Jegou, B., Peake, R., Irby, D. and Kretser, D. (1984). Effects of the induction of experimental cryptorchidism and subsequent orchidopexy on testicular function in immature rats. Biology of Reproduction, 30(1): 179-187.
Kaki, T. and Sofikitis, N. (1999). Effects of unilateral cryptorchidism on contralateral sperm quality quantity and fertilizing capacity. Yonago Acta Medica, 42: 79-86.
Kerr, B., Rich, K. and Fekretser, F. (1979). Alteration of the fine structure and androgen secration of the interstitial cells in the experimental cryptorchid rat testis. Biology of Reproduction, 20(3): 409-422.
Liamara, R., Luis, A. and Fabio, F. (2005). Sperm retrival techniques in rats with suppressed spermatogenesis by experimental cryptorchidism. Human Reproduction, 20(2): 443-447.
Mazen, N.F. and Elnegris, H.M. (2013). Role of coenzyme Q10 in testicular damage induced by acrylamide in weaned albino rats: a histological and immunohistochemical study. The Egyptian Journal of Histology, 36(1): 164-174.
Meistrich, M., Wilson, G. and Porter, K. (2003). Restoration of spermatogenesis in DBCP–treated rats by hormone suppression. Toxicological Sciences, 76(2): 418-426.
Mendis, S. and Kerr, J. (1990). Experimental cryptorchidism in the adult mouse: qualitative and quantitative light microscopic morphology. Journal of Andrology, 11(6): 539-547.
Nishimura, A., Fujimura, M., Hasegawa, F. and Nobuhito, S. (2010). Pharmacokinetic interaction between nifedipine and coenzyme Q10 in rat. Journal of Health Science, 56(3): 310-320.
Pinart, E., Sancho, S. and Briz, M. (1999). Morphologic study of the testis from spontaneous unilateral and bilateral abdominal cryptorchid boars. Journal of Morphology, 239(3): 225-243.
Ren, L., Medan, M. and Ozu, M. (2006). Effects of experimental cryptorchidism on sperm motility and testicular endocrinology in adult male rats. The Journal of Reproduction and Development, 52(2): 219-228.
Royere, D., Guerif, F., Laurent, V. and Hochereau de Reviers, M. (2004). Apoptosis in testicular germ cells., International Congress Series, 1266: 170-176.
Saalu, L., Oluyemi, K. and Omotuyi, I. (2007). α-Tocopherol (vitamin E) attenuates the testicular toxicity associated with experimental cryptorchidism in rat. African Journal of Biotechnology, 6(12): 1373-1377.
Sun, I.L., Sun, E.E., Crane, F.L., Morre, D.J., Lindgren, A. and Low, H. (1992). Requirement for coenzyme Q in plasma membrane electron transport. Proceedings of the National Academy of Sciences of the United States of American, 89(23): 11126-11130.
Vigodner, M. and lewin, L. (2003). Evaluation of damage to the testicular cells of golden hamsters caused by experimental cryptorchidism using flow cytometry and confocal microscopy. International Journal of Andrology, 26(2): 84-90.
Villalba, J.M., Lopez-Lluch, G., Santos-Ocana, C., Rodriguez-Aguilera, J.C. and Navas, P. (2001). Extramitochondrial functions of coenzyme Q. In: Coenzyme Q: molecular mechanisms in health and disease. The American Journal of Clinical Nutrition,73(6):1116-1117
Walter, L., Miyoshi, H., Leverve, X., Bernard, P. and Fontaine, E. (2002). Regulation of the mitochondrial permeability transition pore by ubiquinone analogs. A progress report. Free Radical Research, 36(4): 405-412.
Yin, Y., Hawkins, K. and Dewolf, W. (1997). Heat stress cause testicular germ cell apoptosis in adult mice. Journal of Andrology, 18(2): 159-165.
