Ultrasonographic and histopathological findings of liver and gallbladder of Saanen goat with fascioliasis and dicrocoeliasis
Erfan Nazari
1
(
D.V.M. Graduate, Faculty of Meterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
)
Siamak Alizadeh
2
(
Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Naghadeh Branch, Islamic Azad University, Naghadeh, Iran.
)
Amir Amniattalab
3
(
Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University, Urmia, Iran
)
Keywords: Dicrocoeliasis, Fascioliasis, Histopathology, Saanen goats, Ultrasonography.,
Abstract :
Infection with trematode parasites is common in Saanen goats and these parasites cause damage to liver, lung and intestinal tissues in these animals. This study aimed to evaluate the ultrasonography and pathology of the liver and gall bladder of Saanen goats suffering from fascioliasis and dicrocoeliasis in order used in the interpretation of results and clinical decisions. In this cross-sectional descriptive study used 300 Saanen goats with an average age of 2.7 years and a mean weight of 73.4 kg. First, each of the goats underwent an ultrasound examination, then their liver and gall bladder were examined after slaughter in the slaughterhouse. Also, tissue sections were prepared for histopathological studies. According to the ultrasound results, the most tissue damage was in the middle part of the right lobe and the caudal part of the left lobe of the liver. There was no statistically significant relationship between the sex and age of goats and the location of liver complications (p<0.05). The histopathological results showed that there were eosinophilia remnants of hepatocytes, macrophages and lymphocytes in the migration paths of larvae. The most microscopic damages in the gallbladder included hyperemia, hyperplasia of seromucous glands, and thickening of the mucous layer. Based on the results of this study, ultrasound is a very useful diagnostic method for evaluating fascioliasis and dicrocoeliasis in Saanen goats. The findings of this study can be used in the interpretation of ultrasound images and histopathology results, as well as in clinical examinations and treatment of this goat breed.
• Albogami, B.M.M. (2015). Study on Histopathological Injuries in Ruminants Due to The Effect of Internal Worms at Taif Province: KING ABDULAZIZ UNIVERSITY JEDDAH.
• Alizadeh, S. and Mohammadi, T. (2019). Ultrasonographic Liver Findings in a Sheep Flock Involved in Chronic Fasciolosis.
Iranian Journal of Veterinary Medicine, 13(1): 37-43. • Amniattalab, A. and Manaffar, A. (2015). Histopathology of gallbladder lesions of confiscated livers from cattle slaughtered in urmia abattoir. Veterinary Clinical Pathology The Quarterly Scientific Journal, 9(35): 205-17.
• Borriello, G., Guccione, J., Bosco, A., Pepe, P. and Prisco F. (2021). Fast focus ultrasound liver technique for the assessment of cystic echinococcosis in sheep. Animals, 11(2): 452.
• Borriello, G., Guccione, J., Di Loria, A., Pepe, P. and Prisco F. (2021). Fast focus ultrasound liver technique for the assessment of cystic echinococcosis in sheep. Animals, 11(2): 452.
• Braun, U. and Steininger, K. (2011). Ultrasonographic characterization of the liver, caudal vena cava, portal vein, and gallbladder in goats. American journal of veterinary research, 72(2): 219-25.
• Braun, U., Jacquat, D. and Steininger, K. (2013). Ultrasonographic examination of the abdomen of the goat. II. Liver, spleen, urinary tract and greater omentum. Schweizer Archiv für Tierheilkund, 155(3): 185-95.
• Catalano, S., Symeou, A., Marsh, K.J., Borlase, A., Léger, E. and Fall, C.B. ()2019. Mini-FLOTAC as an alternative, non-invasive diagnostic tool for Schistosoma mansoni and other trematode infections in wildlife reservoirs. Parasites & vectors, 12(1): 1-9.
• Corda, A., Corda, F., Secchi, V., Pentcheva, P., Tamponi, C. and Tilocca, L. (2022). Ultrasonography of parasitic diseases in domestic animals: a systematic review. Animals, 12(10): 125.
• Corrales, J.L,, Cwiklinski, K., Dorey, A., Lalor, R. and Jewhurst, H. (2021). Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA). Veterinary parasitology, 298: 109.
• De Korne, C.M., Van Lieshout, L., Van Leeuwen, F.W.B. and Roestenberg, M. (2023). Imaging as a (pre) clinical tool in parasitology. Trends in Parasitology.
• Dharanesha, N., Muniyellapa, H., Ananda, K., Giridhar, P., Byregowda, S. and Ranganath, G. (2015). Pathological study of acute fasciolosis in goats in Karnataka.
• Dietrich, C., Kabaalioglu, A., Brunetti, E. and Richter, J. (2015). Fasciolosis. Zeitschrift für Gastroenterologie, 53(04): 285-90. • Dinçel, D., Ardicli, S., Samli, H., Ogan, M.M. and Balci, F. (2019). The effect of some environmental factors on growth performance and reproductive traits in Saanen goats. Turkish Journal of Agriculture-Food Science and Technology, 7(10): 1541-7.
• Ghadiri, A., Haji, K.H., Mohammadian, B. and Zahiri, S.M.R. (2007). Ultrasonographic Findings of the Liver in Lori Breed Sheep.Gökdai, A. and Sakarya, E. (2022). Determination of goat milk cost and assessment of factors affecting the profitability of Saanen goat farms in Canakkale province, Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi.
• Gonzalo-Orden, M., Millán, L., Alvarez, M., Sánchez-Campos, S., Jiménez, R., González-Gallego, J., et al. (2003). Diagnostic imaging in sheep hepatic fascioliasis: ultrasound, computer tomography and magnetic resonance findings. Parasitology research, 90: 359-64.
• Hassan, N.M., Farag, T.K., Abu, E.l., Ezz, N.M. and Abou-Zeina, H.A. (2019). Prevalence assessment of gastrointestinal parasitic infections among goats in Giza Governorate, Egypt. Bulletin of the National Research Centre, 43(1): 1-7. • Hawash, Y. (2020). Parasitic Liver Diseases. Liver Diseases: A Multidisciplinary Textbook.:161-72. • Ibrahim, N. (2017). Fascioliasis: systematic review. Adv Biol Res, 11(5): 278-85. • Kahl, A., Von Samson-Himmelstjerna, G., Helm, C.S., Hodgkinson, J., Williams, D., Weiher, W., et al. (2023). Coproscopical diagnosis of patent Fasciola hepatica infections in sheep–A comparison between standard sedimentation, FLUKEFINDER® and a combination of both. Veterinary parasitology, 319: 109. • Kawamoto, Y., Honda, G., Ome, Y., Matsunaga, Y., Uemura, S., Yoshida, N., et al. (2023). Laparoscopic left hepatectomy in a goat as a training model for laparoscopic anatomic liver resection: results of training courses with a total of 70 goats. Surgical Endoscopy, 37(5): 3634-41. • Li, F. and Liu, G. (2024). Fasciola. Molecular Medical Microbiology: Elsevier, 3249-59. • López-Ureña, N.M., Calero-Bernal, R., Vázquez-Calvo, Á., Sánchez-Sánchez, R., Ortega-Mora, L.M. and Álvarez-García, G. (2023). A comparative study of serological tests used in the diagnosis of Toxoplasma gondii infection in small ruminants evidenced the importance of cross-reactions for harmonizing diagnostic performance. Research in Veterinary Science, 165: 105. • Lv, C., Hong, Y., Fu, Z., Lu, K., Cao, X. and Wang, T. (2016). Evaluation of recombinant multi-epitope proteins for diagnosis of goat schistosomiasis by enzyme-linked immunosorbent assay. Parasites & Vectors, 9(1): 1-11. • Mehmood, K., Zhang, H., Sabir, A.J., Abbas, R.Z., Ijaz, M., Durrani, A.Z., et al. (2017). A review on epidemiology, global prevalence and economical losses of fasciolosis in ruminants. Microbial pathogenesis, 109: 253-62. • Moazeni, M. and Ahmadi, A. (2016). Controversial aspects of the life cycle of Fasciola hepatica. Experimental parasitology, 169: 81-9. • Molavi, G., Massoud, J., Gharaguzlo, M., Mahmoudi, M., Solaymani, S. and Khooban, M. (2004). A Survey on the histopathologic changes due to flukes of zoonotic importance in slauther house specimens. Journal of School of Public Health and Institute of Public Health Research, 2(3): 25-36. • Moshnikova, V.S., Gilmour, L.J., Cook, A.K. and Fabiani, M. (2020). Sonographic findings of pinpoint hyperechoic foci in the small intestine, liver, and mesenteric lymph nodes are indicative of canine Heterobilharzia americana infection. Veterinary Radiology & Ultrasound, 61(5): 583-91. • Mukarram Shah, S.M. and Saira Hussain, F. (2023). Molecular Techniques for the Study and Diagnosis of Parasite Infection. Parasitic Infections: Immune Responses and Therapeutics, 176-204. • Rojo-Vázquez, F.A., Meana, A., Valcárcel, F. and Martínez-Valladares, M. (2012). Update on trematode infections in sheep. Veterinary parasitology, 189(1): 15-38. • Signer-Hasler, H., Burren, A., Ammann, P., Droegemueller, C. and Flury, C. (2019). Extent of genomic inbreeding in Swiss sheep and goat breeds. Recherche Agronomique Suisse, (10): 372-9. • Siles-Lucas, M., Becerro-Recio, D., Serrat, J. and González-Miguel, J. (2021). Fascioliasis and fasciolopsiasis: Current knowledge and future trends. Research in veterinary science, 134: 27-35. • Snowden, K.F., Ketzis, J.K. and Trematodes. I. (2021). Greene's Infectious Diseases of the Dog and Cat: Elsevier, 1528-49. • Streeter, R.N. and Step, D. (2007). Diagnostic ultrasonography in ruminants. Veterinary Clinics of North America: Food Animal Practice, 23(3): 541-74. • Yatoo, M.I., Parray, O.R., Ul Haq, R.I. and Mushtaq, M. (2023). Diagnostic Techniques in Goats. Principles of Goat Disease and Prevention, 237-49. • Yi-Zhu, X. and Zhi-Bang, Y. (2010). A case of ectopic fascioliasis in the skin. Tropical doctor, 40(4): 253-4.