The effect of two types of exercise training (continuous and intermittent) and atorstatin on the expression of VEGFR and mir503 genes in aortic tissue of diabetic model rats
Subject Areas : Effect of Physical Activity on Diseases
maryam mosapoor ahmadi
1
,
پروین فرزانگی
2
*
,
Hajar Abbaszadeh
3
,
farzane farokhi@gmail.com
4
1 - Department of Sports Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
2 - Department of Sports Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
3 - Associate Professor, Exercise Physiology Department, Sari Branch, Islamic Azad University, Sari, Iran
4 - Department of Biological Sciences and Technologies, Sari Branch, Islamic Azad University, Sari, Iran.
Keywords: Continuous and intermittent exercises, Atherostatin, Angiogenesis, VEGFR and mir503.,
Abstract :
Introduction: Disruption of natural angiogenesis is one of the key features of many pathological complications related to diabetes. The purpose of this research is to investigate the effect of two types of exercise (continuous and periodic) and the drug atorstatin. on the expression of VEGFR and mir503 genes in the aorta tissue of diabetic model rats. Materials and methods: 40 male rats, which were randomly divided into 8 groups: 1) control, 2) diabetic, 3) diabetic+continuous, 4) diabetic+intermittent, 5) atrostatin, 6) continuous + atrostatin, 7) intermittent + atrostatin and 8) saline. Rats performed two types of continuous and periodic training 5 days a week for 8 weeks. Atrostatin was injected intraperitoneally at a daily dose of 20 mg per kilogram of body weight. Result: VEGFR gene expression in the diabetes group was significantly decreased at the confidence level of 0.05 compared to the control group, and the periodic-supplement group was significantly increased compared to the diabetes group. The expression of the mir503 gene in the diabetes group increased significantly compared to the control group at a confidence level of 0.05, and the continuous, intermittent, continuous-supplement and intermittent-supplement groups decreased significantly compared to the diabetes group (P≤0.05). Conclusion: Based on the obtained results, we see the synergism of exercise training and the drug atorvastatin in increasing VEGFR and decreasing mir503 in the aortic tissue of diabetic rats, which may be effective in reducing the complications of diabetes
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