The effect of bone marrow stem cell transplantation with an aerobic training course on inflammatory and cardiac damage indices in male rats with myocardial infarction
Subject Areas : Effect of Physical Activity on Diseasesshirin Abazari 1 , Seyed Abdollah Hashemvarzi 2 , kamal ranjbar 3
1 - Ph.D Candidate in Exercise Physiology, Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran
2 - Islamic Azad University, Sari Branch
3 - گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه آزاد واحد بندر عباس، هرمزگان، ایران
Keywords: Aerobic traiicning, Stem cell, inflammation, Cardiac damage, Myocardial infarction,
Abstract :
Introduction: Cardiac infarction is one of the most common ischemic heart diseases, and new treatment methods are being considered for its control and treatment. The aim of the present study was to investigate the effect of bone marrow stem cell transplantation with an aerobic training course on inflammatory and cardiac damage indices in male rats with myocardial infarction. Methods: In an experimental study, 60 male rats were divided into 6 groups of 10 rats: healthy control, patient control, sham, training, cell, and training + cell. To induce myocardial infarction, the anterior descending artery was completely blocked using 0.6 silk thread. Bone marrow stem cell extraction, using neobar slide, about 106 cells were injected for each mouse through the tail vein. Rats in the training group performed aerobic exercise with an intensity of 17 m/min for 10 weeks. The research indicators were measured by laboratory kit. Results: The serum levels of TNF-α and IL-10 in the experimental groups decreased and increased, respectively, compared to the patient group, but the changes of TNF-α only in the training + cell group and the changes of IL-10 only in the training and training + cell groups compared to the patient group was significant. CK and LDH damage indices were significantly decreased in the experimental groups of exercise, cell and exercise + cell compared to the patient group. Conclusion:In the present study, bone marrow stem cell transplantation with a course of aerobic training reduced inflammation and reduced heart damage indicators in rats suffering from heart attack.
1. Popov, S. V., Maslov, L. N., Mukhomedzyanov, A. V., Kurbatov, B. K., Gorbunov, A. S., Kilin, M., Azev, V. N., Khlestkina, M. S., & Sufianova, G. Z. (2023). Apelin Is a Prototype of Novel Drugs for the Treatment of Acute Myocardial Infarction and Adverse Myocardial Remodeling. Pharmaceutics, 15(3), 1029. https://doi.org/10.3390/pharmaceutics15031029.
2. Attar, A., Bahmanzadegan Jahromi, F., Kavousi, S. et al. Mesenchymal stem cell transplantation after acute myocardial infarction: a meta-analysis of clinical trials. Stem Cell Res Ther 12, 600 (2021). https://doi.org/10.1186/s13287-021-02667-1.
3. Ranjbar k, Nazari A, Nazem F. Effect of exercise training and L-arginine supplementation on oxidative stress and left ventricular function in rats with myocardial infarction. Physiology and Pharmacology, 2015: 18 (4), 445-454 [Article in Persian]. http://ppj.phypha.ir/article-1-1036-
4. Jorge, L., et al. Cardiac and peripheral adjustments inducedby early exercise training intervention were associated with autonomic improvement in infarcted rats: role in functional capacity and mortality. European heart journal, 2011:32(7): p. 904-912. https://doi.org/10.1093/eurheartj/ehq244.
5. Zahiri, M, Parviz, Sh, , Hosseini, SJ. The use of stem cells in the treatment of heart attack. Journal of Advanced Biomedical Sciences, Fasa University of Medical Sciences, 2019:10(2), 2206-2225. [Article in Persian]. https://orcid.org/0000-0002-5843-5352.
6. Pourheydar B , Shahi M, Farjah GH, Javanmard M, Karimipour M, Atabaki F. Evaluation of apoptosis in hippocampal cells of rat following intravenous injection of bone marrow stromal cells in ischemia-reperfusion model. Studies in Medical Sciences 2014, 25(7): 586-597. http://umj.umsu.ac.ir/article-1-2413.
7. Nagaya N, et al. Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis. Am J Physiol Heart Circ Physiol 2004:287: H2670–H2676. doi:10.1152/ajpheart.01071.2003.
8. Khanabdali R, Rosdah AA, Dusting GJ, Lim SY. Harnessing the secretome of cardiac stem cells as therapy for ischemic heart disease. Biochem Pharmacol [Internet]. 2016;113(January):1–11. doi: 10.1016/j.bcp.2016.02.012.
9. Fisher SA, Zhang H, Doree C, Mathur A, Martin-Rendon E. Stem cell treatment for acute myocardial infarction. Cochrane Database Syst Rev. 2015(9):Cd006536. doi: 10.1002/14651858.CD006536.pub4.
10. Freitas et al. Effects of exercise training and stem cell therapy on the left ventricle of infarcted rats. Rev Port Cardiol. 2019;38(9):649-656. doi: 10.1016/j.repc.2019.02.013. Epub 2019 Dec 5.
11. Bansal A, Dai Q, Chiao YA, Hakala KW, Zhang JQ, Weintraub ST, et al. Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction. Journal of Proteomics 2010;73:2041-9. doi: 10.1016/j.jprot.2010.06.009.
12. Nunes RB, Alves JP, Kessler LP, Lago PD. Aerobic exercise improves the inflammatory profile correlated with cardiac remodeling and function in chronic heart failure rats. Clinics 2013;68:876-82. doi: 10.6061/clinics/2013(06)24.
13. Jalilian J, Behpour N, Hosseinpourdelavar S, Farzaneghi P. Effect of Aerobic Training in Combination with Stem Cells on Inflammatory Biomarker Levels in the Heart Tissue of Rat Model of Osteoarthritis. Scientific Journal of Ilam University of Medical Sciences 2019: 28(1):12-26. doi.org/10.29252/sjimu.28.1.12.
14. Synergistic Effects of Aerobic Exercise after Bone Marrow Stem Cell Transplantation on Recovery of Dopaminergic Neurons and Angiogenesis Markers of Parkinsonian Rats. International Journal of Applied Exercise Physiology, 2016, 1 (5):69-80. https://www.proquest.com/docview/1914263407.
15. Zhou J, Wang Y, Liu Y, Zeng H, Xu H, Lian F1. Adipose derived mesenchymal stem cells alleviated osteoarthritis and chondrocyte apoptosis through autophagy inducing. J Cell Biochem 2018;3:123-8. doi. 10.1002/jcb.27530.
16. Cifuentes D, Rocha L, Silva L. Decrease in oxidative stress and histological changes induced by physical exercise calibrated in rats with osteoarthritis induced by monosodium iodoacetate. Osteoarth Cart 2010; 18:1088-95. doi. 10.1016/j.joca.2010.04.004. Epub 2010 Apr 22.
17. Asadi S, Farzanegi P, Azarbayjani M. Effect of exercise ozone and mesenchymal stem cells therapies on the expression of IL-10 and TNF-α in the cartilage tissue of rats with knee osteoarthritis. Soc Det Health 2018; 4:83-6. doi.org/10.22037/sdh.v4i3.23869.
18. Chirico E, Ding D, Muthukumaran G, Houser S, Starosta T, Mu A, et al. Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart. Physiol Rep 2015; 3:23-9. doi. 10.14814/phy2.12566.
19. Rostami, S., Zamani, A., Rajaeian, B. Effect of resistive-endurance exercises on myocardial tissue creatine kinase isoenzyme, IL-6 and IL-10 serum changes in male cardiovascular patients. Turkish Journal of Sport and Exercise, 2015: 17(1), 7-11. doi:10.15314/TJSE.2015112531.
20. Salimeh, A., Mohammadi, M., Mohaddes, G., & Badalzadeh, R. Protective effect of diosgenin and exercise training on biochemical and ECG alteration in isoproterenol-induced myocardial infarction in rats. Iranian journal of basic medical sciences, 2011: 14(3), 264-274. doi.org/10.22038/ijbms.2011.5004.
21. Kemi, O. J., Høydal, M. A., Haram, P. M., Garnier, A., Fortin, D., Ventura-Clapier, R., & Ellingsen, Ø. Exercise training restores aerobic capacity and energy transfer systems in heart failure treated with losartan. Cardiovascular research, 2007:76(1), 91-99. doi: 10.1016/j.cardiores.2007.06.008 .
22. Kon, M., Kimura, F., Akimoto, T., Tanabe, K., Murase, Y., Ikemune, S., & Kono, I. Effect of Coenzyme Q10 supplementation on exercise-induced muscular injury of rats. Exerc Immunol Rev, 2007:13, 76-88. https://pubmed.ncbi.nlm.nih.gov/18198662/.
23. Bakar, Y., Coknaz, H., Karlı, Ü., Semsek, Ö., Serın, E., & Pala, Ö. O. Effect of manual lymph drainage on removal of blood lactate after submaximal exercise. Journal of physical therapy science, 2015:27(11), 3387. doi: 10.1589/jpts.27.3387. Epub 2015 Nov 30.
24. Chen, Y.-M., Lin, C.-L., Wei, L., Hsu, Y.-J., Chen, K.-N., Huang, C.-C., & Kao, C.-H. Sake Protein Supplementation Affects Exercise Performance and Biochemical Profiles in Power-Exercise-Trained Mice. Nutrients, 2016:8(2), 106. doi: 10.3390/nu8020106.
25. Nunes, R. B., Alves, J. P., Kessler, L. P., & Lago, P. D. Aerobic exercise improves the inflammatory profile correlated with cardiac remodeling and function in chronic heart failure rats. Clinics, 2013:68(6), 876-882. doi: 10.6061/clinics/2013(06)24.
26. Batista, M., Rosa, J., Lopes, R., Lira, F., Martins, E., Yamashita, A., Seelaender, M. Exercise training changes IL-10/TNF-α ratio in the skeletal muscle of post-MI rats. Cytokine, 2010:49(1), 102-108. doi: 10.1016/j.cyto.2009.10.007. Epub 2009 Nov 30.
27. Xu, X., Wan, W., Ji, L., Lao, S., Powers, A. S., Zhao, W., Erikson J.M., Zhang, J. Q. (2008). Exercise training combined with angiotensin II receptor blockade limits post-infarct ventricular remodelling in rats. Cardiovascular research 2008, 78(3):523-32. doi: 10.1093/cvr/cvn028. Epub 2008 Feb 5.