Determination of rhizobium bacteria strain for seed inoculating of bean in north of Fars

Subject Areas : Journal of Plant Ecophysiology

A. Hemmati ¹ , هادی Asadi Rahmani ² , Gh.H. Masoudi ³

1 - عضو هیات علمی میرکز تحقیقات کشاورزی و منابع طبیعی فارس
2 - عضو هیات علمی موسسه تحقیقات آب و خاک
3 - کارشناس مرکز تحقیقات کشاورزی ومنابع طبیعی فارس

Received: 2014-05-28 Accepted : 2014-05-28 Published : 2013-06-01

Keywords:

Abstract :

Nitrogen is necessary for growth in plants. Symbiotic N2-fixation is the most of source nitrogen in legumes. A filed study was conducted to evaluation of 10 rhizobium strains (l-78, l-195, l-192, l-54, l-75, l-30, l-100, l-197. l-120 and l-177) and two nitrogen fertilizers treatments (35 and 75 ppm N) with control treatment (without inoculation and N2 – fertilizer) in yield of bean in 2009-2010. Experimental design was randomized complete block (RCBD) with four replicates. The weight of dry mater, nitrogen in leaf and seed and yield of bean were calculated. In the first year, the results indicated that there was a significant different (at 5 %) in grain yield between treatments. The best seed yield and nitrogen in dry mater were obtained in L-54 strain treatment. This yield was 174 % increased to control treatments. In the second year, the results showed that there are significant different between treatments (at 5 %). The maximum yield was obtained  by     L-77 and L-54 strains with 1595 and 1511 kg/ha respectively. The yield of this treatments were 41 and 33 % increased than control treatment (without inoculate and nitrogen fertilizer). Base of the results in two years of experiment we showed that the highest yield and protein were obtained by rhizobium inoculated of bean