Evaluating the effect of different nitrogen consumption on sesame yield, albumin and globulin proteins electrophoresis

Subject Areas : Journal of Plant Ecophysiology

M.  Kazemian ¹ (kharazmi university)
A.  Majd ² (استاد علوم گیاهی، دانشگاه خوارزمی تهران، تهران، ایران)
P.  Jonobi ³ (استادیار علوم گیاهی، دانشگاه خوارزمی تهران، تهران، ایران)
S.  Kalij ⁴ (استادیار علوم گیاهی، دانشگاه مازندران، ساری، ایران)

Received: 2015-05-08 Accepted : 2015-07-26 Published : 2017-02-19

Keywords: SDS-PAGE, Agronomic Characteristics, densitometry, seed oil,

Abstract :

In order to study the effect of different nitrogen concentrations with urea source (0, 40 and 60 Kg/ha) on sesame (Sesamum indicum L.) yield and amount of albumin and globulin proteins, an experiment arranged in a randomized complete block design was conducted in Babol city, in 2013. In addition, quantitative and qualitative analysis of seed storage proteins were assessed by Bradford and SDS-PAGE methods. Nitrogen fertilizer rates exhibited a significant effect on the plant height, number of branches, number of capsules per plant, capsule length, number of seeds in capsule, 1000 seed weight, seed yield, seed oil and protein percent. Quantitative and qualitative studies showed that albumin and globulin proteins 15 percent decreased and 53 percent increased respectively under nitrogen treatment. Total of two proteins increased by 60 Kg/ha of nitrogen fertilizer consumption in comparison to the control. There is no significant difference between 40 and 60 Kg/ha of nitrogen concentrations in agronomic characteristics. Densitometry analysis showed that albumin protein was minor and globulin protein was the most and the main protein of seed storage total proteins. Generally, the results indicated that a Nas variety of sesame under 40 kg/ha of nitrogen treatment was better in comparison to the control specimen in Agronomic characteristics. In addition, seed oil and seed storage proteins percent enhanced quantitatively and qualitatively under this treatment.