Effect of Trichoderma virens and Piriformospora indica coexistence with Enterobacter sp. on the growth and photosynthetic pigments in pepper (Capsicum annuum L.) plant

Subject Areas : Journal of Plant Ecophysiology

فائزه محمدی کشکا ¹ , Hemmatollah Pirdashti ² , یاسر یعقوبیان ³ , سیده حدیثه بهاری ساروی ⁴

1 - دانشجوی کارشناسی ارشد زراعت، دانشگاه علوم کشاورزی و منابع طبیعی ساری
2 - معاون پژوهشی / پژوهشکده ژنتیک و زیست فناوری کشاورزی طبرستان - دانشگاه علوم کشاورزی و منابع طبیعی ساری
3 - دانشجوی دکتری زراعت، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان
4 - دانشجوی دکتری فیزیولوژی گیاهان زراعی، دانشکده کشاورزی، دانشگاه شاهرود

Received: 2015-04-07 Accepted : 2015-07-16 Published : 2016-12-01

Keywords: Chlorophyll, Carotenoid, Trichoderma, phosphate solubilizing rhizobacteria, mycorrhizae like,

Abstract :

In order to evaluate the Effect of Trichoderma virens and Piriformospora indica coexistence with Enterobacter sp. on the growth and photosynthetic pigments in pepper (Capsicum annuum L.) plant an experiment was conducted during 2015 under controlled conditions. A factorial experiment based completely randomized design with three replicates was used. Factors were two levels of inoculation and non-inoculation of phosphate solubilizing bacteria, Enterobacter sp., and four levels of fungi coexistence including uninoculted control, Trichorerma virens (Tv), Piriformospora indica (Pi) and Tv+Pi. The pepper seeds were inoculated with the mentioned fungi and bacteria before sowing in the sterilized soil. The pots were kept in greenhouse for four weeks and then morphological parameters (leaf number, plant height, root length and leaf area) and aerial parts fresh and dry weights along with chlorophyll a, b, a+b, a/b and carotenoid contents were determined. Results indicated that aboveground fresh weights significantly increased in Pi and Tv+Pi inoculated seeds without Enterobacter as compared to the uninoculated control. Also, coexistence of Tv+Pi and Enterobacter increased root and plant dry weights (up to 58 and 40 %, respectively) and leaf and aerial parts dry weights (more than two times and 93 %, respectively) as compared to the uninoculated control. The maximum amount of carotenoid was recorded in coexistence of Pi and Enterobacter. In conclusion, results of the present study demonstrated that there was a positive effect between the growth promoting fungi, Tv and Pi, and phosphate solubilizing rhizobacteria, Enterobacter, in terms of growth parameters of pepper plant.