Genetic diversity investigation of different grape varieties (Vitis vinifera) using ISSR molecular marker
Subject Areas : Journal of Plant Ecophysiology
A.
Khalkhali
1
(کارشناسی ارشد بیوتکنولوژی کشاورزی دانشگاه ازاد اسلامی واحد دامغان)
Hossein
abbaspour
2
(دانشیار گروه زیست شناسی دانشگاه آزاد اسلامی واحد دامغان)
Keywords: genetic diversity, Molecular markers, Static analysis, grape, Phylogenic Tree,
Abstract :
Grapes is one of the most important horticultural products in the world and Iran and has a wide gnetic diversity, so the purpose of this research was to investigate of the genetic diversity and to asses of introduced grapes cultivars (Vitis vinifera) at the molecular level using ISSR marker. For DNA extraction, it was used modified method of Doyle and Doyle and at the next step, 12 genotypes were examined by 12 primers. Cluster analysis was performed by PopGen32 and SPSS9 softwares. The resulted clustering were divided cultivars into 5 groups by both softwares. In analyzing data, the percentage of polymorphism and the number of polymorphic loci were obtained 96.49 percent and 55 respectively. Cophenetic coefficient is 0.8 for Jaccard coefficient and straw, is indicating a fine fit between the dendrogram and the main similarity matrix. The bands that were Obviously visible were reviewed. Results showed that 353 bands were generated by the markers totally. The size of bands was various between 200 and 3000 bp. The highest similarity was 0.619 for number one (sabz Shah Pasand) and number 7 (currants Khalili Ghouchan) varieties and the least one was 0.205 for number10 (Pir Golli 2) and number 2 (Kashmar Mall) varieties. Because the most amount of bond were observed by primer a (56 bands), this primers was able to determine the genetic gap of Primerrelated varieties better than other primers.
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