Effects of Physiological and Morphological Variation in Gene expression PEAMT1 and PEAMT2 drought resistant and sensitive genotypes of bread wheat
Subject Areas : Journal of Plant Ecophysiology
Mahdi Roozrokh
1
(استادیار گروه زراعت و اصلاح نباتات،واحد کرمانشاه،دانشگاه آزاد اسلامی ،کرمانشاه، ایران)
Keywords: germination, osmotic stress, molecular technologies, Parsi, Pishgam,
Abstract :
To study the response of wheat genotypes to drought and the effects of gene expression, experiments were conducted in a factorial based on randomized complete and randomized complete blocks designs with three replications respectively, in laboratory and greenhouse, during 2015, at Islamic Azad University of Kermanshah,Iran. factors of the designs were included twenty wheat genotypes (Ten drought-resistant and sensitive genotypes) and six levels of osmotic potential. Molecular biology technologies were conducted in faculty of pharmacy ,University of Medical Sciences, Kermanshah, Iran. The results showed that, drought resistant genotypes, Pishtaz,Azar2 and Parsi were Forty percentage, date and rate of germination superior to the drought-sensitive genotypes,Pishgam,Owhadi and Rasad. Root dry weight in the Parsi genotype with 78.99 mg, the maximum amount and more than three times the lowest root dry weight of 24.08 mg was observed in Rasad genotype.in greenhouse High and low limit values of the percentage,date and rate of emergence and plants weight Belonged to drought-resistant genotype,Parsi and drought-sensitive genotype, Pishgam,respectively Increased drought stress in Parsi genotype increased the gene expression TaPMT1,which clearly demonstrates the involvement of this gene at least at the level of gene expression in response to osmotic stress that due to no significant changes expression in sensitive genotype,Pishgam ,can be this gene as a main candidate for further investigation proposed at the level of molecular biology.
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