Population genetic structure of stellate sturgeon (Acipenser stellatus Pallas, 1771) in Ural, Sefidrud and Gorganrud Rivers using microsatellite markers

Subject Areas : New Technologies in Aquaculture Development

Received: 2021-01-11 Accepted : 2021-01-11 Published : 2012-08-22

Keywords: Caspian Sea, microsatellite marker, Genetic population, Keywords: Acipenser stellatus,

Abstract :

Abstract[1] This study represents a larg-scale population genetic analysis of the stellate sturgeon in the Northern (Ural River) and Southern Caspian Sea (estuary of Sefidrud and Gorganrod Rivers-Iran). Totally, 138 individuals of adult stellate sturgeon from three regions were sampled. Fifteen sets of microsatellite primers were developed from lake sturgeon (Acipenser fulvescens) and shovelnose sturgeon (Scaphirhynchus platorynchus) tested on genomic DNA of stellate sturgeon. Allele frequencies, private alleles, observed and expected heterozygosity, Hardy-Wienberg Equilibrium, genetic similarity and distance, FST and RST based on AMOVA were calculated using the Gene Alex software. At this point, the ten successfully used primer sets should be mentioned and polymorphism was observed, while one locus was monomorph and four sets didnt. Analyses revealed that average of alleles per locus was 12.7 (range 8 to 18 alleles per locus in regions). All sampled regions contained private alleles. Average observed and expected heterozygosity were 0.651 and 0.85 respectively. Basis on AMOVA for all FST values among pairs of collections ranged between 0.047 to 0.058 and indicated a significant difference between the three geogeraphic regions (p RST of genotypic differences between these pairs of collections support the existence of different genetic populations along the Caspian Sea coast. *- Corresponding authors; [e-mail]