Evaluation of hydrocare efficacy to reduce the skin bacterial load of beluga (Huso huso) fingerlings and rearing water

Subject Areas : New Technologies in Aquaculture Development

Received: 2020-12-06 Accepted : 2020-12-06 Published : 2009-11-22

Keywords: hydrogen peroxide, Beluga, Disinfection, Keywords: Bacterial load, Hydrocare,

Abstract :

Abstract[1] Hydrocare is a compound of Hydrogen peroxide and sodium percarbonate which applied as a disinfectant. In this research 450 Beluga (Huso huso) fingerlings were kept in 9 veniros, in three replications of control group and treatments.         First treatment was disinfected with 30 g/m3 dosage, second treatment was disinfected with 40 g/m3 dosage for 6 days (30 min) with Hydrocare. Alive samples in rearing water were moved to laboratory .Samples were washed with sterile neutral salt solution to remove adherent bacteria. Skin was removed in sterile condition, and then was homogenized and appropriate dilutions were spread on the surface of TSA (tryptic soy agar) and finally the plates were incubated. For bacterial loading in rearing water, above process was done by the same method .After bacterial growth, CFU (Colony Forming Unit) by means of counting colonies was measured. The mean of skin bacterial flora in the first treatment was 5.08 ±0.39 log CFU/gr and in the second treatment was 4.35±0.35 log CFU/gr and they had a lower amount in comparing with control group with the mean of5.81±0.38 log CFU/gr. In order to One way ANOVA and Tukey HSD tests there is a significant statistical difference. In the study rearing water ,the mean of bacterial flora of rearing water in the first  treatment was4.38± 1.47 log CFU/ml and in the second treatment was3.97±0.45 log CFU/ml and they had a lower amount in comparing with control group with the mean of4.87±1.54 log CFU/ml. In order to One way ANOVA and Tukey HSD tests there is a significant statistical difference.   * Corresponding Author; Email: [e-mail]