Time optimization of heat shock after fertilization for triploid induction in Rainbow trout (Onchorhynchus myksis)

Subject Areas : New Technologies in Aquaculture Development

Received: 2020-12-08 Accepted : 2020-12-08 Published : 2012-02-20

Keywords: Heat shock, Keywords: Rainbow trout, Triploid induction,

Abstract :

Abstract[1] The rainbow trout (Onchorhynchus myksis) is a value fish and has been widely production for human consumption. The sexual development of fish lead to reduce the somatic growth. Thus, production of sterile fish can be useful in aquaculture. Heat shock is a very effective way to optimizing the heat shock treatment time. Heat shock were applied at different times after fertilization (range 10-28 min). Other parameters, in this paper were set as follows: temperature of shock= 26 °C, shock duration= 10 min. the success of triploidy induction was determined by erythrocyte masurement. The results of statistical analysis indicates that the survival rates of treated groups, were lower than those of the control group (p <0.05). Nuclear volume showed a 2.17 times in triploids than diploid. The highest level of triploid induction reached to 80% and were obtained at initiated 28 min after fertilization. *- Corresponding Authors; Email: [e-mail]