Phylogeny of 3 species of Caspian Sea Percids inferred from mitochondrial 12s rRNA gene

Subject Areas : New Technologies in Aquaculture Development

Received: 2021-02-02 Accepted : 2021-02-02 Published : 2013-05-22

Keywords: Caspian Sea, phylogeny, Keywords: Percidae, 12s rRNA gene,

Abstract :

Abstract[1] The family Percidae is one of the most diverse families of freshwater fishes. Previous systematic analysis (that study the genus and species of Percidae) were mainly based on morphological characters. But nowadays molecular data are used for these purposes extensively. The main objective of this study is determining phylogenetic relationships of 3 species of Caspian Sea Percids including: Sander lucioperca, Perca fluviatilis and Sander marinum inferred from mitochondrial 12s rRNA gene sequencing. For this purpose, 3 adult pike perches were collected from Anzali wetland and 3 adult Sander marinum from Caspian Sea. 2 g of each specimen's dorsal fin was removed and stored in 96% ethyl-alcohol. Genomic DNA was extracted and Polymerase Chain Reaction (PCR) was conducted on the target DNA using one pair of 12s rRNA primer. After electrophoresis, PCR product and primers were sent to Genfanavaran Corp. for sequencing. The phylogenic relationships were assessed with Mega 4. The results of this study showed that both S. lucioperca and S. marinum are located in the same cluster and evolutionary distance of these two species is very low. P. fluviatilis with a little more evolutionary distance was located in a distinct cluster. On the other hand, all these three species were located in a separate cluster from Cyprinus carpio which confirm the common ancestor for these three species. As a conclusion, the results of this study showed that sequencing mitochondrial 12s rRNA gene is a reliable method to investigate phylogenetic relationships among Caspian Sea percids. * Corresponding Authors; Email: [e-mail]