Population genetic structure of common kilka (Clupeonella cultriventris) in the South Caspian Sea coastline (Gilan Province) using microsatellite markers
Subject Areas : New Technologies in Aquaculture Development
Keywords: Microsatellite, Clupeonella cultriventris, South Caspian Sea, Keywords: Population genetic,
Abstract :
Abstract This study represents population genetic structure of the common kilka in the Southern Caspian Sea (Gilan Province). Totally, 60 individuals of adult common kilka from two seasons were sampled. Fifteen sets of microsatellite primers were developed from American shad (Alosa sapidissima), Pacific herring (Clupea pallasi), Atlantic herring (Clupea harengus), sardine (Sardina pilchardus) tested on genomic DNA of common kilka. Allele frequencies, the fixation RSTindex and FST on AMOVA test, observed and expected heterozygosity H-W equilibrium, genetic distance and genetic identify were determined at disomic loci amplified from fin tissue samples using GenAlex and uvitec software. At this point only the five successfully used primer sets should be mentioned (Cpa6,Cpa8,Cpa104,Cpa125, AcaC051) and were used to analyze the genetic variation in adultsof the common kilka population. Analyses revealed that average of alleles per locus was 14.4 (range 5 to 21 alleles per locus). Both of sampled seasons contained private alleles. Average observed and expected heterozygosity were 0.153 and 0.888, respectively. All loci significantly deviated from H-W equilibrium. Basis on AMOVA for both RST values among pairs them ranged 0.113 (Nm=1.96) and indicated a significant difference between the two seasons (p≤0.01). The genetic distance between populations was 0.344, which indicates that the genetic difference among the studied populations is pronounced. These results support the existence of different genetic populations along the South Caspian Sea coastline (Gilan Province).
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