Genetic variability and differentiation of Anzali Wetland Pike (Esox lucius) during Spawning Seasons, winter and spring, using microsatellite molecular method
Subject Areas : New Technologies in Aquaculture Development
Keywords: Microsatellite, Anzali Wetland, Esox lucius, Keywords: Pike, Population Genetic,
Abstract :
Abstract Pike, Esox lusius is one of the most valuable commercial species that evaluated the genetic structure in Anzali wetland using microsatellite markers. A total of 60 specimens of adult pikes were sampled from two spawning seasons, winter and spring, in Anzali wetland. Five pairs of microsatellites, tested on the genomic DNA that all loci of microsatellite produced polymorphic bands as polymorphic loci were used to analyze the genetic variation of the pick. Analyses revealed that average of alleles per locus was 10.8 (range 9 to 13 alleles). All sampled seasons contained private alleles. The average observed and expected heterozygosity was 0.913 and 0.883, respectively. The inbreeding coefficient values of five microsatellite loci were negative. With the exception of one loci in spring, all loci significantly deviated from H-W equilibrium (P<0.01). Based on AMOVA, RST and FST values were significant between seasons (P<0.01). The genetic distance between populations was 0.442, which indicates that the genetic difference among the studied populations is pronounced. These results support the existence of different genetic populations in spawning seasons Anzali wetland.
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