Potential βcarotene production from Dunaliella salina of Shahe Lake under salinity stress
Subject Areas : New Technologies in Aquaculture Development
Keywords: Salinity, HPLC, Dunaliella salina, Keywords: βcarotene,
Abstract :
Abstract Carotenoids are widely being used in medical applications, foods, additive substances and cosmetic materials as colors, anti oxidants and anti cancers. Dunaliella as a natural source of mass concentration of carotenoids is considered. In this study, the effect of various salt concentrations of 5, 10, 15, 20, 25, 30, 35 and 40% on production of Beta Carotene in Dunaliella Salina microalgae was evaluated. Dunaliella Salina was isolated from Shahe Lake located in south of Tehran and was purified. The dense growth was performed in altered Janson medium. The flasks containingmedium culture were cultured in suitable conditions. To evaluate beta carotene, the obtained extracts from samples were analyzed by HPLC. The results obtained from analysis of algae samples with different saltiness (0, 5, 10, 15, 20, 25, 30, 35 and 40%) in their culture medium showed a meaningful difference (0.0001) on production amount of beta carotene. The maximum production amount of beta carotene was 0.33 microgram in liter in saltiness of 25%. The observed results from this study showed that by increasing various concentrations in medium culture of this alga until %25 the production of beta carotene increased.
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