The survey of Microalgae (Scenedesmus Sp.) growth within the different medium (TMRL and Z-8+N) culture in Indoor lab condition

Subject Areas : New Technologies in Aquaculture Development

Received: 2020-09-05 Accepted : 2020-09-05 Published : 2020-08-22

Keywords: Keywords: Scenedesmusو TMRL, Z-8+N, Indoor culture,

Abstract :

AbstractAt first microalgae (Scenedesmus sp.) was collected by plankton net (20 microns), samples from Khazarabad area (This research was performed by Caspian research group of fisheries and water pollutants). Samples were transferred into laboratory, isolated and purification by Pipette Pasture method. After the medium culture was made, the stoke of above green algae with primary culture density (7×106 cells/ml) was inoculated to medium culture at 25±2 temperature and 3500±350 LUX light density. This experiment was carried out for the study of growth. In this experiment, it was taken account 2 treatments and 3 replicates. The observation microalga was carried out by light microscope (40X). The tool of counting was utilized by Hemcytometer slide (for five grade samples counting). In addition the number of colony was separately counted. The average counting of colony in medium culture (TMRL&Z-8+N) constitutes five stage which were obtained 6.06×106±1.47×106 and 21.70×106±1.39×106, respectively. The average of single-cell colony with 53.5% and for cell colonies with 39.7% have the most colony growth that was attributed to TMRL and Z-8+N, respectively. The growth rate and growth coefficient was specially measured. These indices in TMRL & Z-8+N were 0.52 and 0.26, respectively. For comparison, the growth value  of above algae was used nonparametric Kroskal-wallis test for total counting, as it showed that there is significant difference from first to five counting in Z-8+N (P=0.009) and TMRL(P=0.027).   But the test of Mann-Whitney had no significant difference about 2 medium cultures (P=0.065). Generally, the comparison of 2 medium cultures was shown that the efficacy of Z-8+N medium culture was higher than TMRL medium culture for microalgae growth Scenedesmus in laboratory condition.