Suitability of formalin-containing fixative for the eggs of Cyprinus carpio and Rutilus frisii kutum

Subject Areas : New Technologies in Aquaculture Development

Received: 2020-01-12 Accepted : 2020-01-12 Published : 2007-05-22

Keywords: Cyprinus carpio, Rutilus frisii Kutum, Keywords: Fixative solution, Egg diameter,

Abstract :

Abstract     The suitability of 12 different formalin-containing fixatives consisted of: Gilson's and Bouin,s flouids, 0.5% to 10% buffered formalin, 0.1% to 1% unbuffered formalin with 0.7% sodium chloride (NaCl) and 0.1% to 1% unbuffered formalin with 0.9% NaCl 1% unbuffered formalin with 0.9% NaCl for the eggs of the Rutilus frisii kutum and Cyprinus carpio were tested.The size of the eggs diameter at  several intervals (0, 0.5, 1, 2, 4, 24, 48, 72, 96, 120-hrs) after fixation were measured. The most suitable of the fixatives were for the wild common carp, 1% unbuffered formalin with 0.9% NaCl, for cultured common carp, 0.1% unbuffered formalin with 0.9% NaCl and 10% buffered formalin for R.frisii kutum, as their level of  swelling or shrinkability were  less than fresh eggs.