Comparing the effect of hormone (GnRH) analogue with anti-Dopamine Domperidone (Ova-fact) through Linpe method with common carp pituitary extract on reproduction indices of grass carp (Ctenopharyngodon idella)

Subject Areas : New Technologies in Aquaculture Development

Received: 2021-02-03 Accepted : 2021-02-03 Published : 2014-05-22

Keywords: Reproduction, Ctenopharyngodon idella, Keywords: Broodstock, Pituitary gland, Ovaprim,

Abstract :

Abstract[1] Grass carp, Ctenopharyngodon idella is one of the important species of Chinese carp. In the present research, pituitary gland of common carp (Cyprinus carpio) and Ovaprim hormone was used for inducing spawning in carp broodstock. 40 broodstocks were selected with the average weight and overall length of 8090±228.27 gr. and 904±20 ml, respectively. The experiment was carried out using 2 treatments (including, pituitary treatment and Ovaprim treatment) and 20 broodstocks in each control (10 male broodstocks and 10 female broodstocks). In treatment 1, 4 and 2 mg/kg of pituitary gland extract hormone were used for female and male broodstock fishes, respectively. In treatment 2, 0.5 ml/kg and 0.25 ml/kg of Ovaprim synthetic hormone were used for female and male broodstock fishes, respectively. Water temperature during the reproduction period was 22.5±1 °C. Injection of pituitary and Ovaprim in the broodstock was performed intramuscularly in one stage. In Ovaprim hormone treatment, 100 percent of carp female broodstocks reacted positively to the injection, whereas spawning success in pituitary injection was 80 percent and no significant different was seen in the groups (P>0.05). Latency period of 13-14 hours was variable in the treatments. Fertilization percentage in Ovaprim treatment was 89.8±1.87, showed a significant difference as compared with pituitary hormone which was 64±3.25 (P<0.05). Hatchability, survival rate of larvae, extraction of total eggs obtained in Ovaprim treatment were 84.9±1.66, 81.2±2.82, 520295±23306, the relevant percentages obtained in pituitary treatment were 66.3±3.87, 65.4±3.1, 408250±17883 and production rate 64069.5±681 and 50238.6±555, which showed a significant difference (P<0.05). According to the results obtained in connection with reproduction indices, Ovaprim hormone showed a better efficiency than pituitary for inducing spawning.   * Corresponding Authors; Email: [e-mail]