Study of different salinity effect on cells density of Nannochloropsis oculata
Subject Areas : New Technologies in Aquaculture Development
Keywords: Iran, Salinity, Keywords: Nannochloropsis oculata, cells density,
Abstract :
Abstract This study was conducted to consider the semi-intensive culture of Nannochloropsis oculata at Gomishan workshop located in the Golestan Province in the autumn 2003 and winter 2004. All experiments were done in f/2 medium and cell abundance was provided in five different logarithmic scale salinity 20, 22.5, 25, 28.5, and 32 grams per liter. The initial density was 5×106 cells ml-1 and at the end of the sixth day the number of Nannochloropsis oculata was counted by a Neo-Bar Lam and microscope; light absorption in 750nm was determined by Spectrophotometer method. According to this study at the end of the sixth day, 20 and 28.5 grams per liter salinity with 75×106 and 38×106 respectively have maximum and minimum cell abundance. In media culture with salinity of 20 and 28.5ppt, absorption rate was calculated 1.077 and 0.508 respectively. There was no significant difference (P<0.05, Sig=0.217) in the different salinities (Kroscal-Valis test). This study indicated the salinity of 20gram per liter is an effective salinity for the culture of the mentioned micro algae.
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