The of minimum inhibitory concentration of growth in the epidermal mucus of Caspian roach (Rutilus rutilus caspicus) fed dietary supplemented with different levels of vitamin C by disc diffusion method

Subject Areas : New Technologies in Aquaculture Development

Received: 2020-09-02 Accepted : 2020-09-02 Published : 2020-02-20

Keywords: vitamin C, Minimum inhibition concentration, Keywords: Epidermal mucus, Caspian roach (Rutilus rutilus caspicus),

Abstract :

Abstract[1] This study was conducted to evaluate the effects of different levels of vitamin C on the minimum inhibitory concentration of mucus of Caspian roach, Rutilus rutilus caspicus. Six hundred and sixty fish were obtained from Sijaval aquaculture center (Torkemen Port) and after 2 weeks acclimation, were distributed in 12 tanks with 4 treatments and 3 replicate (55 fish in each tank). Vitamin C with 0, 1000, 1500 and 2000 mg/ kg diet doses were added to food for 60 days. At the end of trial mucus were diluted with distilled water that was 200, 100, 50, 25 and 12.5 micro liter/ ml liter and they were put against gram-positive bacteria Streptococcus faecium, Micrococcus luteus and Gram-negative bacteria Serratia marcescens and Escherichia coli. The results by disc diffusion method were showed that 200 µl/ml concentration of mucus have minimum antibacterial activity against two strains of bacteria. Dilution of mucus decreased the antibacterial activity. Inhibition zone diameter in fish fed with different levels of vitamin C significant increase compared to control group (p < 0.05). The results of this study were showed that increase of mucus immunity by means of disease decrease was obtained with vitamin C as an immune stimulant that is influence affects. * Corresponding authors; [e-mail]