Production of hydrolyzed protein from viscera of beluga Huso hoso by Alcalase enzyme
Subject Areas : New Technologies in Aquaculture Development
Keywords: Response Surface Method, Hydrolyzed protein, Huso hoso, Keywords: Alcalase enzyme,
Abstract :
Abstract Response Surface Method (RSM) was employed for optimizing the temperature, enzyme quantity, and time in order to production of hydrolyzed protein form viscera of beluga Huso hoso. Thus, the effects of three independent variables including temperature, time and enzyme quantity were investigated on hydrolization rate as a surface respond. On the basis of three dimensions graphs, the optimum condition for temperature, time and enzyme quantity consisted of 50 ˚C, 120 min and 1%, respectively. The hydrolyzedprotein contained 66% protein and 1.34% lipid by using Alcalase 2.4 L enzyme. The results of this study revealed that the proportion of lipid content has been dramatically decreased in hydrolyzedprotein rather than initial row materials.
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