Determination acute toxicity of the Captan fungicide )LC5096h) and its effects on blood factors fingerling grass carp (Ctenopharyngodon idella)
Subject Areas : New Technologies in Aquaculture Development
Keywords: Ctenopharyngodon idella, Blood indices, LC5096h, Keywords: Captan fungicide,
Abstract :
Abstract The effects of Captan poison that use large quantity in agricultural fields, especially in the north of Iran, was studied on 180 pieces 3-5gr fingerlings Ctenopharyngodon idella in this research, aim to determine the 50% lethal concentration (LC50) in 96 hours. These experiments carried out in static condition and based on (O.E.C.D, 2001) standard method in 4 days, and were controlled water physicochemical parameters including; pH, total hardness, dissolved oxygen and temperature. During experiments, reaction and behavior of the fingerlings exposed to poison was surveyed. Early experiment for find of mortality ration with released the number of 10 fish per aquarium were achieved into a 20lit aquarium and continuously aerated. Lc50 value of Captan poison in 96 hours was calculated 0.9 mg/l for fingerlings C.idella and also M.A.C value (Maximum Allowable Concentration value) was determined 0.09 mg/l. Then, to determining effect Captan poison on blood indices with keeping to observation standard condition, fish in laboratory placed in concentration 25%, 50%, 75% of LC50 96h Captan ordering (0.225mg/l, 0.45mg/l, 0.675mg/l) for 7 days and was proceed blood sampling. Results showed decreasing values of lymphocyte, hemoglobin, red blood cells, white blood cells, hematocrit, eosinophil (except from treatment 50%), MCHC and increase the values of MCH (except from treatment 25%), MCV(except from treatment 25%) and monocyte, neutrophil.
_||_