Population Genetic Structure of Neogobius caspius (Eichwald, 1831) in the South Caspian Sea using PCR-RFLP Marker

Subject Areas : New Technologies in Aquaculture Development

Received: 2020-11-02 Accepted : 2020-11-02 Published : 2008-11-21

Keywords: RFLP, PCR, Genetic population, Keywords: Caspian Sea, Neogobius caspius,

Abstract :

Abstract Neogobius caspius is a small benthic fish that is native of the Caspian Sea. The importance of this fish is because of its role as a main food resource of the Sturgeon fish. The genetic diversity of N.caspius population in the Caspian Sea was studied using PCR-RFLP technique. A total of 135 samples of N.caspius were collected from costal line in the north Caspian Sea, including specimens from costal of Anzali and Chalus. Genomic DNA was extracted by phenol-chloroform method and then was amplified using a pair primer of cytochrom b gene, 2 tRNA gene and the control region sequences by a thermal cycler. R: D2(5'-CCGGAGTATGTAGGGCATTCTCAC-3') F: CY1(5'-YYTAACCRRGACYAATGACTTGA-3') 12 restriction enzyme were used to digest the target gene region including: AluI - Alw26I(BsmAI) - BSeNI(BSRI)- DraI- MboI- RsaI – TasI – HincII- BsuRI(HaeIII) - Bsh1285I - Hin1I- Bsu15I. Digested PCR products were observed by silver staining method followed by polyacrilamid gel electrophoresis (PAGE). The results showed the same pattern among the species. There was no polymorphism and no differentiation in population in the Neogobius caspius fish in the southern coast of Caspian Sea.