The effect of some cryoprotectants on the hatching rate of sturgeon embryos (Acipenser persicus)
Subject Areas : New Technologies in Aquaculture Development
Keywords: Hatching rate, Keywords: Cryopreservation, Cryoprotectant toxicity, Sturgeon embryos,
Abstract :
Abstract Cryopreservation of fish embryo is replacement of cryoprotectants with water in side embryo. Cryopreservation of fish embryos requires an optimal concentration of cryoprotectants inside all embryo compartments. At this study toxicity of cryoprotectants, fish embryos were examined in stage (6 hours after fertilization).In the present study, to evaluate the cryoprotectant toxicity was determined in sturgeon embryos at morula stage (6 hours after fertilization). From permeable cryoprotectants, methanol, dimethyl sulfoxide and combination of dimethyl sulfoxide with ethylene glycol in concentrations (0.5, 0.75, and 1 M) and non-permeable, sucrose was used. Embryos treated in three stage and for each stage about 5 minutes from low to above molarity, and the embryos were incubated until hatching. There were no significant differences between cryoprotectants differences concentrations, in terms of hatching rate in each of three treatment compared with the controls (P>0.05 and under Duncan). However among the permeable cryoprotectants, combination of dimethyl sulfoxide with ethylene glycol had the highest toxicity for sturgeon embryos. But embryos better tolerated differences concentrations of dimethyl sulfoxide than two other cryoprotectants and less sensitive than.
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