Effects of dietary ethanolic Extract of Propolis, a honeybee product, on immune parameters of common carp (Cyprinus carpio)

Subject Areas : New Technologies in Aquaculture Development

Received: 2021-02-02 Accepted : 2021-02-02 Published : 2014-02-20

Keywords: challenge, Propolis, immune response, Keywords: Cyprinus carpio,

Abstract :

Abstract[1] In this study, the effects of bee Propolis extract on humoral immune response of common carp Cyprinus carpio were investigated. For this purpose, the 300 common carp fingerlings (intial weight 50±10 g) were divided into four groups and fed with a control diet and a diet containing. 0.1%, 0.25%, 0.5% ethanol extract of Propolis for 60 days. The blood samples were provided on days 0, 20, 40 and 60 and Lysozyme activity, bactericidal activity, total protein, albumin and globulin were measured. At the end of the trial fingerlings were exposed to the bacterial pathogen, Aeromonas hydrophila and mortality was recorded for 10 days. The results indicate that the diet 0.5% ethanol extract of Propolis increased significantly lysozyme activity in total protein, globulin (P<0.05). Bacterium Aeromonas hydrophila mortality after bacterial encounter test also treated 0.5% Propolis extract showed a significant decreasing compared to control while mortality in 0.25% and 0.1% was not significant (P>0.05). The results of this experiment suggest that suitable concentrations of Propolis extract in the diet to stimulate humoral immunity and increased resistance to testing encounter bacterial is concentration of 0.5%. * Corresponding Authors; Email: [e-mail]