Effect of adding earth worm to diet on hematological parameters and hepatic enzymes of bester (Acipenser ruthenus× Huso huso)

Subject Areas : New Technologies in Aquaculture Development

زهره رحمتی‌پرست ¹ , حبیب وهاب‌زاده ² , محمود محسنی ³

1 - گروه شیلات، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران
2 - گروه شیلات، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران
3 - سازمان تحقیقات، آموزش و ترویج کشاورزی، مؤسسه تحقیقات علوم شیلاتی کشور، مؤسسه تحقیقات بین‌المللی تاسماهیان دریای خزر، رشت

Received: 2017-10-15 Accepted : 2017-10-15 Published : 2016-05-21

Keywords: blood parameters, Earthworm, Hepatic enzymes, Bester (Acipenser ruthenus ×Huso huso),

Abstract :

This study was conducted to investigate the effects of replacement of dietary fish meal with earthworm in Bester (Acipenser ruthenus × Huso huso) diet.  450 fish (350±35 g) were adapted with new condition and distributed into 9 concrete tanks and were reared with three experimental treatments (100%pelleted diet as a control, 15% live food + 85% pelleted diet and 30% live food + 70% pelleted diet) throughout 8 weeks. After 8 weeks of rearing, the blood samples were collected to assess haematological parameters of fish in the various treatments. Result showed significant differences in blood parameters such as white and red blood cell, Hb, Htc, MCH, Neutrophil, Lymphocyte and Monocyte (P<0.05), But there were no significant difference in and mean corpuscular haemoglobin concentration (MCHC;%) and eosinophil.  In this study significant differences were observed in cholesterol, triglyceride, alkaline phosphates, SGPT and SGOT, levels but there is no significant differences were observed in total protein, albumin, urea and blood nitrogen between treatments. According to the results, adding 15% earthworm into pelleted diet improved blood parameters and can be replaced in pelleted diet.