Whole and differential leukocyte count of Golden mullet (Liza auratus) during breeding migration
Subject Areas : New Technologies in Aquaculture Development
Keywords: Leukocyte, Keywords: Golden mullet, Differential count, Breeding migration,
Abstract :
Abstract During 22nd of October 2004 to 5th of January 2005; along western part of Mazandaran province (in 4 stations) 30 golden mullets with different sizes were caught by beach seine. Fishes were anesthetized by MS222 then blood samples were taken. Blood smears were prepared and utilized for differential leukocyte counts (lymphocytes, neutrophils, monocytes, eosinophils, special granulocytic cell and immature cells). Also, whole leukocyte counts conducted in improved Neubauer. Number of leukocytes per each mm3 of blood, in different length classes was 43225±15550 in class I (22-27cm), 46766±16680 in class II (27-33 cm) and 44250±15870 in class III (33-40cm). Lymphocytes percentages in mentioned classes were 98%, 97% and 97% respectively. The average of neutrophils in class I was 2% and for two other classes was 3%. Now monocytes and eosinophiles were observered. There was no significant difference between leukocytes count in length classes (P.0.05). This is the characteristic of a normal population of fish, which all are in the same maturation stage or with one stage difference. There were more small lymphocytes than large lymphocytes in Golden mullet. The number of nuclei in neutrophils was about 2-3 and nuclei in 4 or 5 pieces were rarely seen. Neutrophiles were larger than lymphocytes. Nuclei of lymphocytes were darker, more intense and basophilic. Considering viral infections in Golden mullet during last 2-3 years in Caspian Sea leukocytes count decreased to 50% of normal condition. Actually it’s a leucopenia.
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