The effects of 4- nonylphenol compound on the genetic diversity in the prelarvae of the Iranian sturgeon (Acipenser persicus)
Subject Areas : New Technologies in Aquaculture Development
Keywords: genetic diversity, Microsatellite, Keywords: Acipenser persicus, Hetrozygocity,
Abstract :
Nonylphenols havecomprehensive usage in plastic, cleaning products, resins and stabilizers in industry and via sewage permeate to aquatic ecosystems. The study demonstrated the effects of the 4-nonylphenolon microsatellites in the prelarvae of the Iranian sturgeon (Acipenser persicus) as biomarkers to investigate their genetic diversity. 480 prelarvae of the Iranian sturgeon in the 4 treatments and 3 replications (two sublethal concentrations 40 and 70 µg/l of the 4-nonylphenol, control and sham) was applied in the ten days three periods. The experiment was carried out semi-static condition. Evaluation genetic diversity of the prelarvae's Iranian sturgeon for four pairs of the microsatellites markers (AfuG68b, AfuG63, AfuG241 & LS-68) carried out in the above four concentrations. Finally, the minimum number of alleles observed were 2 (AfuG68b) and the maximum number of alleles observed were 4 (AfuG63) in the 70 µg/l of the 4-nonylphenol. The minimum heterozygocity observed was 0.733 (AfuG68b) and the maximum hetrozygocity observed was 1(AfuG241). The minimum hetrozygocity expected was 0.511 (AfuG68b) and the maximum hetrozygocity expected was 0.644 (AfuG63). At the fourtreatments, the medium hetrozygocity expected &hetrozygocity observed 0.852 and 0.583, respectively. The number of effective allels were increased in the 70 µg/lat the AfuG63 locus than control. Therefore, the genetic diversity was increased in the environmental with 4-nonylphenol compounds.
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