Determination of LC50 and Some Histopatological Changes Due to Sevin in Common carp "Cyprinus carpio” Juveniles
Subject Areas : New Technologies in Aquaculture Development
Keywords: Acute toxicity, histopathological changes, Common carp (Cyprinus carpio), Keywords: Sevin,
Abstract :
Abstract The acute toxicity and histopathology of common carp fish exposed to sevin were assessed following the O.E.C.D. Guideline and performed statically in 20±2˚C.In acute toxicity experiments the mean body weight of the juveniles was 3.63±0.26 g. The 96h LC50 value of sevin for common carp juveniles was 14.187 mg/l Therefore the MAC value of sevin in natural waters for “Cyprinus carpio” was 1.4187 mg/l. According to the table of sorting the toxicity of insecticides, sevinn was toxic for common carp .The following clinical symptoms were observed in this study consisted of lordosis and neural paralytic syndrom in fish exposed to this pesticide. Some abnormal reactions such as losing the balance and swimming in a half circle , expressive pigmentation mainly on the dorsal part and block of respiration movements were seen in these juveniles. Histopathological experiments performed on control and treatment group of common carp fish with 3.8±0.42 g mean body weight after 96h exposure to sevin in concentration lower than LC50 96h (10mg/l). Samples were taken from tissues of liver, kidney, gills, spleen, gut in both treatmentl and control groups. Sever hyperplasia, edema, inflamation and degenaratin of lamellas were observed in tissues of gills in experimental group. Also vacuolization, necrosis and multifocal hemosiderosis were seen in liver and spleen. More than this lesions, there was kidney`s tissue necrosis and enlargement of bowman`s capsule In kidney`s tissue. The result revealed that, it is concluded that histopathology of these juveniles exposed to environmental contaminants may provide useful information to target organs and mechanism of toxic substance actions.
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