E Effects of mercury on sperm motility and testis structure of Caspian kutum (Rutilus frisii kutum) by using in vitro experiments

Subject Areas : New Technologies in Aquaculture Development

Received: 2020-06-24 Accepted : 2020-06-24 Published : 2018-11-22

Keywords: Spermatozoa, Keywords: Caspian Sea, Kutum, Mmercury,

Abstract :

Abstract[1] Mercury is a toxic element enters into the Caspian Sea that can accumulate in aquatic animals’ organ. The aim of this study was to evaluate the testis tissue changes and estimating spermatozoa motility of kutums. At first, by using in vitro culture, testis with 10-5, 10-6 and 10-7 M of Hgcl2 solution, incubated for 3 and 6 days and in second experiment, kutums spermatozoa motility evaluated, after exposing with 0, 0.01, 0.1, 1, 10, 100 and 1000 mg/l of Hgcl2 solutions. The harmful effects of mercury on spermatogenesis and testis structure, with the increasing of concentration and exposed time, increased. As the inhibition of spermatogenesis and necrotic germ cells were more visible in 10-5 M in 6th day. Sperm motility parameters decreased significantly (P<0.05), with the increasing of mercury concentration and spermatozoa stopped in 100 and 1000 mg/l. According to these results, amounts of injurious effects on testis and spermatozoa motility had direct relationship with time and mercury concentration, and continuous pollution in fish environment would have irreparable injuries in future.   *- Corresponding authors; [e-mail]