Determination of Neogobius kessleri gorlap population genetic structure in Iranian coasts of the Caspian Sea (Guilan and Golestan) by microsatellite markers
Subject Areas : New Technologies in Aquaculture Development
Keywords: Caspian Sea, Microsatellite, Neogobius kessleri gorlap, Keywords: Genetic variation,
Abstract :
Genetic diversity of Neogobius kessleri gorlap poplution was examined by 100 samples from Iranian coasts of the Caspian Sea (Guilan and Golestan). Genomic DNA was extracted from fin tissue by phenol-chlorophorm method and its concentration was 50 to 100 nanogram. PCR was performed using 6 microsatellite primers. PCR products were resolved through vertical non-denaturing 8% polyacrylamide gels electrophoresis. The amplified microsatellite loci were visualized through silver staining. The results showed that each of 6 primers were polymorphism in Neogobius kessleri gorlap . The mean of observed and effective allel number was 11.333 and 7.066 respectively in Golestan coast and 10.200 and 5.737 in Guilan coast. Also the mean of observed and expected heterozygosity was 0.571 and 0.812 respectively. It was also seen that specimens from two regions (exception locus NG71, NG111 and NG215 in Golestan coast) were not in Hardy-Weinberg Equibrium in all of the loci. Based on Analysis of Molecular Variance (AMOVA) FST was 0.052 between Golestan and Guilan coasts. Also Nm value was 4.521 between two regions. Genetic distance was 0.634 between specimens from Guilan and Golestan coasts. As result, there is a significant genetic divergence between some of samples. Therefore, two genetic group of Neogobius kessleri gorlap were identified in Iranian coasts of the Caspian Sea (Guilan and Golestan coasts).
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