Determination the lethal concentration (Lc50 96 H) of chloropyrifos and diazinon on (Rutilus Rutilus Caspicus)

Subject Areas : New Technologies in Aquaculture Development

Received: 2020-06-24 Accepted : 2020-06-24 Published : 2018-05-22

Keywords: diazinon, Rutilus rutilus caspicus, LC5096h, Chloropyrifos, Keywords: Insecticide,

Abstract :

Abstract      The survey acute toxicity of Chloropyrifos and Diazinon toxins that use large quantity in agriculture fields specific in Golestan province studied onRutilus rutilus caspicusfingerlings (1-3g) in this research, aim to determine the 50% lethal concentration (LC50) in 96 hours. These experiments carried out in static condition and based on  TRC,1984 standard method in 4 days, and controlled of the effective water physico – chemical factors that having ranges pH =7–8.2 , DH=205 mg /l (CaCO3), DO=7mg/l and Temp=26oC .During experiments reaction and behaviour of the fingerlings exposure of toxin seen carefully.Early experiments for find of mortality range with released in to an 20lit aquarium (10 fingerling of 1-3g in aquvarium) and continous aeration.Finally experiments with 5 treatment,3 repetition and 1 blank.On the base of obtained results by Probit program version 1.5  LC50 value of Chloropyrifos and Diazinon in 96 hours were 0.016 and 12.81 mg/l on Rutilus rutilus caspicus respectively, and also Mac(Maximum allowable concentration) value of this toxins were determined 0.0016 and 1.281 mg/l for Rutilus rutilus caspicus respectively.