Comparable Antibacterial Effects of Zinc and graphene Oxide Nanoparticles on Urinary Calculi obtained after Percutaneous Nephrolithotomy
Subject Areas : Applied Microbiology
1 - Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Keywords: Graphene Oxide, Zinc Oxide, nanoparticles, urolithiasis, Bacterial infection.,
Abstract :
Background: science and industry have focused on preparing nanoparticles (NPs). Due to the resistance of bacteria to antibiotics, the need to replace effective antimicrobials with fewer side effects is overwhelming. Objectives: The aim of this study was to synthesize Zinc and graphene oxide NPs and investigate the antimicrobial effects on bacteria isolated from urinary stones. Methods: Cases of urolithiasis extracted from 45 patients with staghorn stones through percutaneous nephrolithotomy were included in the study. Urinary stone culture was performed. Isolation and identification of Bacteria were done by standard microbiological techniques. Zinc and graphene Iron oxide NPs were synthesized and minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) were evaluated for isolates. Results: FeSEM, zeta test and DLS of nanoparticles showed the purity of synthesized zinc oxide and graphene oxide nanoparticles. The size of 70 nm for graphene oxide and 500 nm for zinc oxide was obtained. The antibacterial effects of nanoparticles showed that zinc oxide at a concentration of 1000ppm had maximum inhibition due to the diameter of the non-growth halo of 25mm and graphene oxide with the same concentration of 18mm was obtained for Escherichia coli bacteria. The results of MBC of zinc oxide nanoparticles 228µg/ml and graphene oxide 300µg/ml and MIC of zinc oxide nanoparticles 180µg/ml and graphene oxide 250µg/ml were obtained. Conclusion: Zinc oxide and graphene oxide nanoparticles had antibacterial effects on gram-positive and gram-negative bacteria isolated from kidney stones of patients and can be used in the treatment of infections caused by kidney stones.