Isolation and antibiotic sensitivity of E. coli K99 isolates from diarrheic calves at Fars province

Subject Areas : Medical Microbiology

Zahra Shams ¹ (Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran)
Yahya Tahamtan ² (Department of Bacteriology, Razi Vaccine and Serum Research Institute, Shiraz, Iran)
Mohammad Kargar ³ (Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran)
Seyed Mohammad Hosein Hoseini ⁴ (Department of Bacteriology, Razi Vaccine and Serum Research Institute, Shiraz, Iran)
Seyed Ali Pourbakhsh ⁵ (Department of Bacteriology, Razi Vaccine and Serum Research Institute, Shiraz, Iran)

Received: 2009-10-17 Accepted : 2010-01-15 Published : 2010-01-01

Keywords: Escherichia coli K99, antibiotic susceptibility, Calves,

Abstract :

Background and Objective: Diarrhea in newborn calves caused by enterotoxigenic Escherichia coli K99 is one of the important problems is the animal industrial husbandry. In this study we try to find the frequency of E. coli k99 in diarrheal newborn calf, and also determine antibiotic susceptibility of isolates. Material and Methods: 312 rectal swabs of diarrheal calves were obtained in various parts of Fars province. The samples were investigated using biochemical test. All E. coli isolates tested for antibiotic susceptibility using disc diffusion method. Results: of 312 samples, 298 E. coli isolates were obtained, from which the slide agglutination method 13 isolates were identified Escherichia coli k99. These isolates were susceptible to enrofloxacin, nalidixic acid and flomocuen antibiotics. Also, there is some wide range of antibiotics resistance to in Escherichia coli k99. Conclusion: The prevalence of Escherichia coli k99 with diarrhea in calves was 4 / 3% in Fars province which compared to other reports ranking in the lower range.

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