LAMP detection of Cryptococcus neoformans in AIDS patients
Subject Areas : Molecular Microbiology
Mohammad Hassan Shahhosseiny
1
(Department of Microbiology, Islamic Azad University, Qods branch, Tehran, Iran)
Alireza Rezaei Amirabadi
2
(Department of Microbiology, Islamic Azad University, Karaj branch, Alborz, Iran)
Jalil Vand Yousefi
3
(Department of Microbiology, Islamic Azad University, Karaj branch, Alborz, Iran)
Mohammad Ghahri
4
(Department of Biology, Imam Hossein University, Tehran, Iran)
Kayhan Azadmanesh
5
(Department of Virology, Pasteur Institute, Tehran, Iran)
Elham Moslemi
6
(Department of Biology, Islamic Azad University, Tehran east Branch, Tehran, Iran)
Keywords: LAMP, Cryptococcus neoformans, AIDS,
Abstract :
Background and Objective: Cryptococcus neoformans is opportunistic capsulated yeast that infects immunocompromised individuals. Infection occurs due to inhalation of the agent and common infection form is meningitis. Several methods are common to detect this life threthening agent but none of them have well accepted in sensitivity, specificity and rapidness. In this research the effort has been on installation and optimizing the LAMP assay in order to detect the agent itself and its distribution importance of Cryptococcus neoformans in serum specimens of AIDS patients. Material and methods: Atotal number of 34 serum specimens were collected from 34 AIDS patients. After applying sensitivity and specificity tests for the installed technique, optimization of the assay was performed on the specimens. The specimens got inactivated and after steps of DNA extraction, 6 designated primers for the target gene (ura5) were applyed for detection of Cryptococcus neoformans by using the LAMP method. Results: In this study 3 specimens of the total number of 34 AIDS patients were reported positive by using LAMP technique. This it reveals the importance of Cryptococcal infection in AIDS patients. Conclution: Loop-mediated isothermal amplification is an applicable, sensitive, quick and lucrative method for detection of Cryptococcus neoformans. Diagnostic laboratory can use this method without requiring any special instruments.
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