The prevalence of genes encoding Leukocidins in both methicillin resistant and methicillin sensitive strains of Staphylococcus aureus strains isolated from burn patients in Taleghani hospital, Ahvaz
Subject Areas : Molecular Microbiology
Hajar Hoveizavi
1
(Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Azar Dokht Khosravi
2
(Department of Microbiology, School of Medicine, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran)
Zahra Farshadzadeh
3
(Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences,
Ahvaz, Iran)
Keywords: MRSA, MSSA, Bi-component leukocidin, Burn infection,
Abstract :
Bakground and Objective: Various strains of Staphylococcus aureus produce different bi-component toxins such as LUKE/D and PVL. This strains of S.aureus strains are associated with severe skin diseases, fatal pneumonia and osteomyelitis with high morbidity and mortality. The aim of this study was to determine the prevalence of genes encoding Leukocidins in Staphylococcus aureus strains resistant and sensitive to methicillin isolated from burn patients in, Ahvaz. Materials and Methods: This cross sectional study was performed on scar specimens of 203 burn patients hospitalized in Taleghani hospital. All samples were evaluated by traditional culture method and standard biochemical tests for detecting of S. aureus strains. After extracting DNA, mecA, PVL and LUKE/D genes were detected by using the polymerase chain reaction technique (PCR). Results: S. aureus strains were isolated from 95 cases out of total studied samples (46.8%), betwwn them 83 strains (87.36%) were mecA positive. The prevalence of PVL and LUKE/D genes in MRSA strains were 7.23% and 66.26% respectively, while this prevalence were 33.3% for both genes in MSSA strains. Conclusion: Regarding to the high frequency of PVL and LUKE/D genes in MRSA strains, also to severe and lethal diseases caused by these bacteria, early diagnosis and proper treatment must be considered for the prevention of disease progress.
1. Woodford N, Livermore DM. Infections caused by Gram-positive bacteria: a review of the global challenge. J Infect. 2009; 59(S1): S4-S16.
2. Kaiser ML, Thompson DJ, Malinoski D, Lane C, Cinat ME. Epidemiology and risk factors for hospital-acquired methicillin-resistant Staphylococcus aureus among burn patients. J Burn Care Res. 2011; 33(3): 429-34.
3. Warren DK, Guth RM, Coopersmith CM, Merz LR, Zack JE, Fraser VJ. Epidemiology of methicillin-resistant Staphylococcus aureus colonization in a surgical intensive care unit. Infect Control Hosp Epidemiol. 2006; 27(10): 1032-40.
4. Fuchs PC, Kopp J, Häfner H, Kleiner U, Pallua N. MRSA- retrospective analysis of an outbreak in the burn centre Aachen. Burns. 2002; 28(6): 575-8.
5. Jeremić LP. Frequency of methicillin-resistant Staphylococcus aureus (MRSA) in healthy nasal carriers in Pomoravlje district. Acta Medica Medianae. 2010; 49(1): 33-6.
6. Diederen BMW, Kluytmans JAJW. The emergence of infections with community-associated methicillin resistant Staphylococcus aureus. J Infect. 2006; 52(3): 157-68.
7. Grundmann H, Aires-de-Sousa M, Boyce J, Tiemersma E. Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. Lancet. 2006; 368(9538): 874-85.
8. von Eiff C, Friedrich AW, Peters G, Becker K. Prevalence of genes encoding for members of the staphylococcal leukotoxin family among clinical isolates of Staphylococcus aureus. Diag Microbiol Infect Dis. 2004; 49(3): 157-62.
9. Morinaga N, Kaihou Y, Noda M. Purification, cloning and characterization of variant LukE-LukD with strong leukocidal activity of staphylococcal bi-component leukotoxin family. Microbiol Immunol. 2003; 47(1): 81-90.
10. Barrio MB, Rainard P, Pre´vost G. LukM/Luk F'-PV is the most active Staphylococcus aureus leukotoxin on bovine neutrophils. Microbes Infect. 2006; 8(8): 2068-74.
11. Clark J. A brief review of Panton-Valentine leucocidin producing staphylococcal infections in the intensive therapy unit. Current Anaesthesia & Critical Care. 2008; 19: 330-2.
12. Cunnington A, Brick T, Cooper M, Danin J, Hunt D, Jeanes A, Kearns AM, Nolan M, Lyall H. Severe invasive Panton-Valentine Leucocidin positive Staphylococcus aureus infections in children in London, UK. J Infect. 2009; 59(1): 28-36.
13. Meyer F, Girardot R, Piemont Y, Pre´vost G, Colin DA . Analysis of the specificity of Panton-Valentine Leucocidin and gamma-hemolysin F component binding. Infect Immun. 2009; 77(1): 266-73.
14. Forbes BA, Sahm DF, Welssfeld AS. Baily and Scotts Diagnostic Microbiology, 12 Ed, Mosby Inc., St. Louis.
15. Strommenger B, Kettlitz C, Werner G, Witte W. Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. J Clin Microbiol. 2003; 41(9): 4089-94.
16. Merlino J, Watson J, Rose B, Beard_pegler M, Gottlieb T, Bradury R , Harbour C. Detection and expression of methicillin/oxacillin resistance in multidrug-resistant and non-multidrug-resistant Staphylococcus aureus in Central Sydney, Australia. J Antimicrob Chemother. 2002; 49(5): 793-801.
17. Havaei SA, Ohadian Moghadam S, Pourmand MR, Faghri J. Prevalence of genes encoding bi-component leukocidin among clinical isolates of methicillin–resistant Staphylococcus aureus. Iran J Publ Health. 2010; 39(1): 8-14.
18. Arciola CR, Baldassarri L, Von Eiff C, Campoccia D, Ravaioli S, Pirini V, Becker K, Montanaro L. Prevalence of genes encoding for staphylococcal leukocidal toxins among clinical isolates of Staphylococcus aureus from implant orthopaedic infections. Int J Artif Organs. 2007; 30(9): 792-7.
19. Graves SF, Kobayashi SD, Braughton KR, Diep BA, Chambers HF, Otto M , Deleo FR. Relative contribution of Panton-Valentine leukocidin to PMN plasma membrane permeability and lysis caused by USA300 and USA400 culture supernatants. Microbes Infect. 2010; 12(6): 446-56.
20. Gang RK, Sanyal SC, Bang RL, Mokaddas E, Lari AR. Staphylococcal septicemia in burns. Burns. 2000; 26(4): 359-66.
21. Santucci Z, Gobara S, Santos CR, Fontana C, Levin AS. Infections in a burn intensive care unit: experience of seven years. J Hosp Infect. 2003; 53(1): 6-13.
22. Bagdonas R, Tamelis A. Analysis of burn patients and the isolated pathogens. Lithuanian Surgery. 2004; 2(3): 190-3.
23. Turnidge JD, Nimmo GR, Pearson J, Gottlieb T, Collignon PJ, Australian Group on Antimicrobial Resistance. Epidemiology and outcomes for Staphylococcus aureus bacteraemia in Australian hospitals, 2005-6: report from the Australian Group on Antimicrobial Resistance. Commun Dis Intell. 2007; 31(4): 398-403.
24. Li J, Zeng H. A study of MRSA in a burn unit with PCR fingerprinting. Zhonghua shao shans za zhi. 2001; 17( 2): 88-90.
25. Stefani S, Varaldo P. Epidemiology of methicillin-resistant staphylococci in Europe. Clin Microbiol Infect. 2003; 9(12): 1179-86.
26. Fatholahzadeh B, Emaneini M, Aligholi M, Gilbert G, TaheriKalani M, Jonaidi N, Eslampour MA, Feizabadi MM. Molecular characterization of methicilin-resistant Staphylococcus aureus clones from a teaching hospital in Tehran. Jpn J Infect Dis. 2009; 62(4): 309-11.
27. Japoni A, Alborzi A, Orafa F, Rasouli M, Farshad SH. Distribution patterns of methicillin resistance genes (mecA) in Staphylococcus aureus isolated from clinical specimens. Iran Biomed J. 2004; 8(4): 173-8.
28. Rahbar M, Yaghoobi M, Fattahi A. Comparison of different laboratory methods for detection of methicillin resistant Staphylococcus aureus. Pak J Med Sci. 2006; 22(4): 442-5.
29. Ekrami A, Samarbafzade AR, Alavi M, Kalantar E, Hamzeloi F. Prevalence of methicillin resistant Staphylococcus species isolated from burn patients in a burn center, Ahvaz, Iran. Jundishapur J Microbiol. 2010; 3(2): 84-91.
30. Vahdani P, Saifi M, Aslani MM, Asarian AA, Sharafi K. Antibiotic resistance patterns in MRSA isolated from patients admitted in ICU infectious ward. Tanaffos. 2004; 3(11): 37-44.
31. Melles DC, Gorkink R, Boelens H, Snijders SV, Peeters JK, Moorhouse MJ, van der Spek PJ, van Leeuwen WB, Simons G, Verbrugh HA, van Belkum A. Natural population dynamic and expansion of pathogenic clones of Staphylococcus aureus. J Clin Invest. 2004; 114(12): 1732-40.
32. Aires-ed-sousa M, Conceicao T, Lencastre H. Unusually high prevalence of nosocomial Panton-Valentine leukocidin positive Staphylococcus aureus isolates in Cape Verde Islands. J Clin Microbiol. 2006; 44(10): 3790-3.
33. Holmes A, Ganner M, McGuane S, Pitt TL, Cookson BD, Kearns AM. Staphylococcus aureus isolates carrying Panton-Valentine Leucocidin genes in England and Wales: frequency, characterization, and association with clinical disease. J Clin Microbiol. 2005; 43(5): 2384-90.