Role of Rapid Urase test in comparison with PCR for Helicobacter Pylori Infection diagnosis

Subject Areas : Diagnostic Microbiology

Mohammad Kargar ¹ , Sayed Hadi Razavizadegan ² , Kavoos Eshraghian ³ , Mohammad Yaghob Rajpout ⁴ , Sadegh Ghorbani-Dalini ⁵ , Mehdi Kargar ⁶

1 - Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
2 - Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
3 - Jahrom University of Medical Science, Jahrom, Iran
4 - Jahrom University of Medical Science, Jahrom, Iran
5 - Islamic Azad University, Jahrom Branch, Jahrom, Iran, Member of Young Resercher Club
6 - Department of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, Iran

Received: 2009-04-14 Accepted : 2009-05-15 Published : 2009-04-01

Keywords: PCR, H. pylori, Rapid urease test, ureC,

Abstract :

Background and aim: H. pylori is a spiral shaped gram negative non invasive bacterium which causes peptic ulcer and has an important role in gastric carcinoma and MALT associated lymphoma. The aim of this research is to assess sensitivity and specificity of Rapid urease test in comparison with PCR. Material and Methods: 30 patients (test group) with peptic ulcer disease (PUD) and 30 patients (control group) with Non-Ulcer disease (NUD) were chosen from two central of endoscopy in  Fars province during 2006. Endoscopy samples were examined by PCR and Rapid urase methods. In this study, PCR test considered as golden standard test. Results: The assessment of infection sensitivity and specificity ,PPV and NPV Rapid urease test  for the test group were detected 76.67%, 80.76%,69.23% , 85.92%, 60.65% and in  control group were 46.67%,52.94%,68.18%, 41.62% and 77.17% respectively. Conclusion: This study indicated that the Infection’s rate, sensitivity and specificity of Rapid Urase test acceptable in patients who are not under treatment. But the Infection’s rate, sensitivity and specificity of Rapid Urase test not acceptable in patients who are on Proton Pump Inhibitors (PPI) and treatment whit antibiotic. So complementary tests in these patients are recommended.