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Manuscript ID : JMW-2108-1995 (R1) Visit : 151 Page: 78 - 87

20.1001.1.20083068.1401.15.50.5.7

Article Type: Original Research

The evaluation of the effect of gold nanoparticles on the expression of (MexA/B) efflux pump genes from the RND family in multidrug resistant Pseudomonas aeruginosa strains

Subject Areas : Bacteriology

Nehleh Falahati 1 (Islamic Azad University, Jahrom branch Department of Microbiology Ph.D. student Iran)
Houshang Jamali 2 (Islamic Azad University, Jahrom Branch Department of Microbiology Faculty of Sciences Iran)
Mohammad Kargar 3 (Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Farshid Kafilzadeh 4 (Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)

Received: 2021-12-29 Accepted : 2022-04-30 Published : 2022-06-05

Keywords: Gold nanoparticles, Pseudomonas aeruginosa, Multidrug resistance, mexA/B,

Abstract :

Background and purpose: Pseudomonas aeruginosa has several protein pumps with which it puts antibiotics out. This has led to the formation of multidrug resistance. The aim of this study was to investigate the effect of gold nanoparticles on the expression of mexA/B genes in multidrug       resistant Pseudomonas aeruginosa strains. Materials and Methods: In this cross-sectional descriptive study, 74 suspected samples of        Pseudomonas genus were collected and phenotypic and genotypic tests were used to screen     Pseudomonas aeruginosa.Then, anibiogram test using 11 antibiotics from different classes was performed by the use of CLSI 2020 standard tables and the disk diffusion method.The frequency of mexA/B genes was evaluated by polymerase chain reaction method and after determining the minimum inhibitory concentration of gold nanoparticles, the effect of gold nanoparticles on the expression of mexA/B genes was evaluated using SYBER Green-Real Time PCR technique. Results: In this study (67.57%) 50 isolates of Pseudomonas aeruginosa were identified. The     highest and lowest levels of antibiotic resistance were related to azteronam (98%) and cefpime (26%). The prevalence of mexA/B genes was respectively  MexA (74%), mexB (70%) and      mexA/B (58%) and also 12% lacked both genes. Gold nanoparticles showed growth inhibitory effect at MIC ≥ 50ppm and at 25 ppm concentration with ciprofloxacin, showed inhibitory effect on the expression of mexA/B genes. Conclusion: The results of this study showed that gold nanoparticles can inhibit the expression of mexA and mexB genes in multidrug-resistant Pseudomonas aeruginosa. Due to the fact that gold nanoparticle does not have toxic effects on eukaryotic cells, it can be important in the treatment process of this group of bacteria.  

References:

References

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  27. Khare T, Mahalunkar S, Shriram V, Gosavi S, Kumar V. Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli. Environmental                 Research.2021; 199:1-12
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  28. Ahmed FY, Aly UF, Abd El-Baky RM, WalyNGFM.Effectoftitaniumdioxidenanoparticles on the expression ofeffluxpump and quorum-sensing genes in MDR Pseudomonas aeruginosa isolates. Antibiotics. 2021; 10(625):1-15
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  29. Sharif R, and Amini K. Effect of Iron Oxide Nanoparticles and Probiotic Bifidobacterium Bifidum on MexA Gene Expression in Drug Resistant Isolates of Pseudomonas aeruginosa. Research in Medicine.2019;43(3): 118-123[ in Persian ]
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  30. AbdolhosseiniM,Zamani H, Salehzadeh1A. Synergistic antimicrobial potential of ciprofloxacin with silver nanoparticles conjugated to thiosemicarbazide against ciprofloxacin resistant Pseudomonas aeruginosa by attenuation of MexA-B efflux pump genes.Biologia. 2019; pp: 1-6
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  31. Dashtizadeh1Y, MoattariA, GorzinAA.Phenotypic and genetically evaluation of the prevalence of efflux pumpsand antibiotic resistance in clinical isolates of Pseudomonas aeruginosa among burned patients admitted to GhotbodinShiraziHospital.Journal of Microbial World. 2014; 4(2): 118-127 [ in Persian ]
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_||_

References

  1. Tillotson GS, Zinner SH. Burden of antimicrobial resistance in an era of decreasing susceptibility. Expert Review of Anti-infective Therapy.2017; 15:663–676
    2.
  2. TorresMR, Slate AJ, Ryder SF, Akram M, Iruzubieta3 CJC, Whitehead KA. Ionic gold demonstrates antimicrobial activity against Pseudomonas aeruginosa strains due to cellular   ultrastructure damage. Archives of Microbiology.  2021;203:3015–3024
    3.
  3. Li X, Robinson SM, Gupta A. Functional gold nanoparticles as potent antimicrobial agents against multidrug-resistant bacteria. ACS Nano.2014; 8(10): 10682–10686
    4.
  4. Dreier J and Ruggerone P. Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa. Frontiers in Microbiology. 2015;8(660):1-21
    5.
  5. Ali SG, Ansari MZ, Alzohairy MA. Biogenic gold nanoparticles as potent antibacterial and antibiofilmnano-antibiotics against Pseudomonas aeruginosa. Antibiotics.2020;9(100): 1-13
    6.
  6. Cao Q.HaemophilusparasuisCpxRA two-component system confers bacterial tolerance to environmental stresses and macrolide resistance. Microbiol Research. 2018; 206:177–185
    7.
  7. Housseini B Issa K, Phan G, Broutin I. Functional mechanism of the efflux pumps transcription regulators from Pseudomonas aeruginosa based on 3D structures. Frontiers in Molecular Biosciences. 2018; 5(57):1-20.
    8.
  8. Fernando D, Kumar A. Resistance-nodulation-division multidrug efflux pumps in gram-negative bacteria: role in virulence. Antibiotics.2013; 2(1):163–181
    9.
  9. Hasani A, MadhiM,Gholizadeh P. Metal nanoparticles and consequences on multi‑drug resistant bacteria: reviving their role. SN Applied Sciences.2019;1(360): 1-13
    10.
  10. FernandezL, and HancockRE.Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance. Clinical Microbiology Reviews.2012;25: 661–681
    11.
  11. Ding F, Songkiatisak P, Cherukuri PK, Huang T, Xu XHN. Size-dependent inhibitory effects of antibiotic drug nanocarriers against Pseudomonas aeruginosa. ACS Omega. 2018;3:1231−1243
    12.
  12. Skwarecki AS,MilewskiS,SchielmannM,Milewska MJ. Antimicrobial molecular nanocarrier-drug conjugates. Nanomedicine.2016;12: 2215−2240
    13.
  13. Pelgrift RY, Friedman AJ. Nanotechnology as a therapeutic tool to combat microbial resistance.Advanced Drug Delivery Reviews. 2013;65(13):1803–1815
    14.
  14. Imlay JA. Diagnosing oxidative stress in bacteria: not as easy as you might think. Current Opinion in Microbiology.2015; 24:124–131
    15.
  15. Ashraf S,Pelaz B, del Pino P. Gold-based nanomaterials for applications in nanomedicine. Topics in Current Chemistry.2016;370: 169–202
    16.
  16. Jiang X, and Jia Y. Adjust the surface modification of gold nanoparticles to adjust their antibacterial properties.the Chinese Chemical Society Conference.2016;pp: 142-143
    17.
  17. Su C,HuangK,LiHH,Lu YG, Zheng DL. Antibacterial properties of functionalized gold nanoparticles and their application in oral biology. Journal of Nanomaterials .2020;pp:1-13
    18.
  18. Clifford R, Milillo M, PrestwoodJ. Detection of bacterial 16S rRNA and identification of four clinically important bacteria by Real-Time PCR.Plos One.2012;7(11):1-6
    19.
  19. Aghamollaei H, MoghaddamMM, KooshkiH, HeiatM,MirnejadR, BarziNS.Detection of Pseudomonas aeruginosa by a triplex polymerase chain reaction assay based on lasI/R and gyrB genes. Journal of Infection and Public Health. 2015; 8(4): 314-322
    20.
  20. Weinstein M.P. Lewis JS. BobenchikAM, et al. Performance Standards for Antimicrobial Susceptibility Testing. 30thed. CLSI supplement M100.Clinical and Laboratory Standards Institute.2020;  pp: 42-45
    21.
  21. Izadi Pour JahromiS, Mardaneh J, Sharifi A, Pezeshkpour V,Behzad-Behbahani, A,SeyyediN,Dehbidi GR, Manzouri L, Pourmasoudi M, Khoramrooz SS. Occurrence of a multidrug resistant pseudomonas aeruginosa strains in hospitalized patients in southwest of Iran: Characterization of resistance trends and virulence determinants. Jundishapur Journal of Microbiology,2018;pp: 1-11
    22.
  22. Guitor AK, Wright GD. Antimicrobial Resistance and Respiratory Infections. Chest.2018; 154: 1202–1212
    23.
  23. El SayedZaki M, Elewa A, Al-Kasaby NM. Molecular study of efflux genes in Pseudomonas aeruginosa isolated from clinical samples.International Journal of Current Microbiology and Applied Sciences.2017; 6(7): 4549-4556
    24.
  24. JamaliS,Shahid M,Farrukh S, Singh A, Khan HM. Molecular characterization of genes encoding AmpC beta-lactamases in clinical isolates of Pseudomonas and Acinetobacter species. Journal of Pharmaceutical Sciences.2015;5: 048–051
    25.
  25. Singh P, Pandit S, Beshay M, Mokkapati VRSS. Anti-biofilm effects of gold and silver nanoparticles synthesized by the Rhodiolarosea rhizome extracts. Artificial cells, nanomedicine and Biotechnology. 2018;pp:1-14
    26.
  26. Khan F, Manivasagan P, Lee JW, Pham DTN, Oh J, Kim YM.Fucoidan-stabilized gold nanoparticle-mediated biofilm inhibition, attenuation of virulence and motility properties in Pseudomonas aeruginosa PAO1.Marine drugs.2019;pp: 1-19
    27.
  27. Khare T, Mahalunkar S, Shriram V, Gosavi S, Kumar V. Embelin-loaded chitosan gold nanoparticles interact synergistically with ciprofloxacin by inhibiting efflux pumps in multidrug-resistant Pseudomonas aeruginosa and Escherichia coli. Environmental                 Research.2021; 199:1-12
    28.
  28. Ahmed FY, Aly UF, Abd El-Baky RM, WalyNGFM.Effectoftitaniumdioxidenanoparticles on the expression ofeffluxpump and quorum-sensing genes in MDR Pseudomonas aeruginosa isolates. Antibiotics. 2021; 10(625):1-15
    29.
  29. Sharif R, and Amini K. Effect of Iron Oxide Nanoparticles and Probiotic Bifidobacterium Bifidum on MexA Gene Expression in Drug Resistant Isolates of Pseudomonas aeruginosa. Research in Medicine.2019;43(3): 118-123[ in Persian ]
    30.
  30. AbdolhosseiniM,Zamani H, Salehzadeh1A. Synergistic antimicrobial potential of ciprofloxacin with silver nanoparticles conjugated to thiosemicarbazide against ciprofloxacin resistant Pseudomonas aeruginosa by attenuation of MexA-B efflux pump genes.Biologia. 2019; pp: 1-6
    31.
  31. Dashtizadeh1Y, MoattariA, GorzinAA.Phenotypic and genetically evaluation of the prevalence of efflux pumpsand antibiotic resistance in clinical isolates of Pseudomonas aeruginosa among burned patients admitted to GhotbodinShiraziHospital.Journal of Microbial World. 2014; 4(2): 118-127 [ in Persian ]
    32.

 

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