The evaluation of the effect of gold nanoparticles on the expression of (MexA/B) efflux pump genes from the RND family in multidrug resistant Pseudomonas aeruginosa strains
Subject Areas : Bacteriology
Nehleh Falahati
1
(Islamic Azad University, Jahrom branch
Department of Microbiology
Ph.D. student
Iran)
Houshang Jamali
2
(Islamic Azad University, Jahrom Branch
Department of Microbiology
Faculty of Sciences
Iran)
Mohammad Kargar
3
(Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Farshid Kafilzadeh
4
(Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Keywords: Gold nanoparticles, Pseudomonas aeruginosa, Multidrug resistance, mexA/B,
Abstract :
Background and purpose: Pseudomonas aeruginosa has several protein pumps with which it puts antibiotics out. This has led to the formation of multidrug resistance. The aim of this study was to investigate the effect of gold nanoparticles on the expression of mexA/B genes in multidrug resistant Pseudomonas aeruginosa strains. Materials and Methods: In this cross-sectional descriptive study, 74 suspected samples of Pseudomonas genus were collected and phenotypic and genotypic tests were used to screen Pseudomonas aeruginosa.Then, anibiogram test using 11 antibiotics from different classes was performed by the use of CLSI 2020 standard tables and the disk diffusion method.The frequency of mexA/B genes was evaluated by polymerase chain reaction method and after determining the minimum inhibitory concentration of gold nanoparticles, the effect of gold nanoparticles on the expression of mexA/B genes was evaluated using SYBER Green-Real Time PCR technique. Results: In this study (67.57%) 50 isolates of Pseudomonas aeruginosa were identified. The highest and lowest levels of antibiotic resistance were related to azteronam (98%) and cefpime (26%). The prevalence of mexA/B genes was respectively MexA (74%), mexB (70%) and mexA/B (58%) and also 12% lacked both genes. Gold nanoparticles showed growth inhibitory effect at MIC ≥ 50ppm and at 25 ppm concentration with ciprofloxacin, showed inhibitory effect on the expression of mexA/B genes. Conclusion: The results of this study showed that gold nanoparticles can inhibit the expression of mexA and mexB genes in multidrug-resistant Pseudomonas aeruginosa. Due to the fact that gold nanoparticle does not have toxic effects on eukaryotic cells, it can be important in the treatment process of this group of bacteria.
References
_||_
References